T UTORIAL 5
B ACTERIAL TAXONOMY
Compare and contrast phenetic and phylogentic trees of 13 genera of Enterobacteriaceae
The dendrogram is based on the biochemical properties presented in the table provided.
Only the positive (+, 90-100%) and nega
MEDIA AND KOCH'S POSTULATES
Define and give two examples for each of the following:
Explain the characteristics, use and limitations of the following
Overview/ learning outcomes
After review of this lecture you will be able to:
9th May 2014
Room G14D Samuels building
Explain how metabolic diversity leads to microbial
interactions (link to
Introduction to viruses
Professor Peter White
Level 2 Rm 263A
What is a virus?
How common are they?
Classification and genomes
1. Record the details about the sample you are using (source, date of collection). Place about
half a teaspoonful of soil in the empty flask. Add the Nitrogen-Free Broth up to about the 50
2. Incubate the flask at 30C for 2-5 d
Energy Photo/Chemo light energy vs. oxidation of chemical compounds
Electrons - Litho/Organo inorganic substances / organic molecule as electron source
Carbon source Auto/Hetero uses CO2 or reduced organic materials
Azotobacter are relatively large for bacteria (12 m in diameter). They are usually oval, but may take various forms
from rods to spheres. In fresh cultures, cells are mobile due to the numerous flagella. Later, the cells lose their
Evolution on trial
o 2005 kitzmiller vs dover school area district
York county oennsylvania
Intelligent design requirement in high school
o Irreducible complexity
Human immune system
ID proposes that intermedi
Microbiology week 5 lectures
What is the human microbiome?
All microorganisms (the microbiota) that live on and in humans. More specifically the
collection of microbial genomes that contribute to the broader genetic portrait, or
Microbiology lecture 3
Cell membrane and wall
Plasma or cytoplasmic membrane
Absolute requirement for all cellular organisms.
Cells major point of contact with the environment.
Encloses the cytoplasm of cells
Membranes contain proteins
Week 8 Tutorial: Top Ten - No6 Variola (27/4/16 or 28/4/16)
Introduction: Orthopoxvirus is a genus of double stranded virus. The genus contains the
Variola virus responsible for smallpox. It has had an enormous impact on humankind both
Lectures week 7
The kingdom fungi is one of the odles ta nd largest groups of living organisms
It is a monophyletic group
All modern fungi can be traced back to a single ancestral organism which diverged from a
Energy coming chemical or light
Phototrophs use light-trapping molecules to harvest energy directly from light photons
Light photosynthetic pigments excited state energy used to reduce CO2 or generate ATP
Week 7 Tutorial: Top Ten - No5 Penicillium
Introduction: Penicillium is a eukaryotic genus containing over 300 species. The
Penicillium genus is a massive clan of fungal lineages of enormous importance in
medicine, agriculture, ecology and foo
Week 9 Tutorial: Top Ten - No7 Saccharomyces
Poster child of the Eukaryota
Introduction: Saccharomyces is a eukaryotic genus with over 25 species.
Saccharomyces cerevisiae was domesticated thousands of years ago for the production
of bread, wine and beer.
Viral Infections - 2
HCV and HIV
Prof Peter White
Properties of Viruses
- can pas through 0.22m filters
- 20 - 350nm in size
Totally dependent on living cells for replication and
Possess only one species of
To understand the following:
! Definitions of microbial death
! The need to control microbial growth
! Factors that affect microbial death
! Methods of sterilisation
A single death is
Discuss the use and limitations of moist heat, dry heat, chemical and filtration sterilisation
Select the most appropriate method and conditions for sterilisation of the following items.
T UTORIAL 6
R EPORT WRITING
This tutorial is designed to help you prepare and write the scientific report for your isolation
The report is to be written in the form of a scientific paper.
There is a very good web link (see below) that
What is the difference between chemolithrophs and chemoorganotrophs?
Give microbial examples for both nutritional classes and indicate electron donors
for each case?
What is anaerobic respiration?
MICROBIOLOGY OF FOOD 1
L ist the factors, which you think, might influence the number and the type of
microorganisms in different foods.
From the following microorganisms select the two most likely
1. Evaluate possible risk factors for the family.
2. Identify at least 3 sources of infection or food-borne disease.
3. Name 5 possible microbial pathogens lurking in (or near) this lunch. Include at least one protozoan and one virus.
4. What advice do yo
What is soil?
Microbial diversity in soil
What is the diversity of soil?
What are common and well-understood soil
What are their functions?
What is soil?
Profile of mature soil
Review the main strategies available to
microorganisms for obtaining energy and carbon
Fundamentals of redox-based energy generation
and key electron donors/ acceptors
Key examples that demonstrate the metabolic
diversity unique to mic
Importance of fungi in human disease
Species fungi >70,000
Approx 300 cause disease in man and animals
Professor Hazel Mitchell
School of Biotechnology and Biomolecular Sciences
Classification of fungi on basis of
A bacterial cell
What are the main structural element of
a bacterial cell?
What are the molecular properties of
these cell structures?
What are their main functions?
A/Prof Julian Cox
Associate Dean (Education)
Faculty of Science
Food Microbiology ?
and beverages, and
What is microbiology ?
What is microbiology
The study of microorganisms
organisms too small to be seen without magnification
typically viable as single-cell organisms
"It has long been an axiom of mine that the little things are infinitely the most
To understand the following:
!What is microbial growth?
!The physical factors, which influence
!Nutritional requirements and classification
Microbial processes II
Revise of anaerobic respiration and key electron
How fermentation works
How the metabolic processes of microorganisms
result in more complex microbial successions