Dr. Michael Bulger, MRBX 1-11108
Dr. Yi-Tao Yu
Dr. Peter Gibbs
Series of lectures/short talks
3 write ups; schedule exible depending on progress and other
3 quizzes; READ AHEAD. One assignment.
13 February 2013
This is intended as a guide for those new to the
The rest of the talk is essentially a walk-through of
the approach you can take, using the type of
freeware that you can use in the pri
the Process, the Tools, and the Applications
Dr. Mark D. Platt
Techniques in Biochemistry and Molecular Biology
April 8, 2014
Office: MRB G9625
E-mail: [email protected]
What is Proteomics?
Purification of Recombinant Proteins
Stefanie DeVito, Munger Lab. March 18th, 2014
Objectives of lab this week
We will understand and use the tools of protein
purification to purify a protein, using affinity
Next week: We will u
Enzymes Lab Quiz 1 Name i ' 2014
1. Briey, why do we use 0nitrophenyl[Sgalactoside instead of the
natural substrate, lactose in our experiments?
2. Enzyme 1 has a Km of 5 yM while enzyme 2 has a Km 2 75 yM.
Which is more efficien
Quiz Feb 11, 2014
1. Give two advantages of sequence protocols that use Taq DNA polymerase. (2 points)
Any two of:
Less hands on operation
No alkaline denaturation step for double-stranded templates
Less starting template
INTRODUCTION TO DNA MANIPULATION AND ANALYSIS: QUIZ #1
1. List two strategies of primer design that can minimize the synthesis of nonspecific products (2 pts).
Any of the following accepted:
G or C at 3 end of primers (GC clamp)
Maintain moderate GC conte