BMB 801: Introduction to Molecular Biology
Any of these three texts provide suitable background:
Molecular Biology of the Gene, Watson et al.
Genes (Lewin; or other authors in the latest edition)
Molecular Biology of the Cell, Alberts et al.
Biology 4110 Biomedical Technology and Molecular
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PRINT your name on the front of this cover page and on the back of the other pages.
12. Indicate the type of DNA element involved in RNA polymerase II transcription. (6 pt)
a) Prevents position effects on transgenes
b) Bound by the general transcription machinery
c) Bound by sequence specific transcription
Short Answer. Answer all of the following. Use only the space provided.
9. You are working with a newly isolated exotic eukaryotic species discover a new RNA
polymerase. The RNA polymerase initiates transcription only from a single, highly
19. A biochemist replaces the DNA-binding domain of the yeast Gal4 activator protein
with the DNA-binding domain from the bacterial LexA repressor, and finds that the
engineered protein no longer regulates transcription of the GAL genes in yeast.
Multiple Choice. Answer all of the following. Circle the letter corresponding to
each correct answer. More than one answer may be correct for each question. (4
pt each, 32 pt total, partial credit will be given)
1. Which of the following activities are ca
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5. You are interested in studying the
mechanism of action of a particular short
interfering RNA. For your experiments, you
transfect cells with a lucifierase reporter
gene. You also transfect th
Analysis. Answer two of the three following questions (18-20). Answer all parts of
the two questions that you have selected. When asked to explain your answer, do
more than just restate the results (10 pt each)
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16. You are interested in studying the regulation of your favorite gene
(YFG). Based on preliminary data, you hypothesize YFG is activated by
transcription factors X and Y and that these factors interact through a DNA
element located at -300 to -200 of YF
BMB 801 Fall 2013
Welcome to Biochemistry & Molecular Biology 801!
The focus of this course is: Cellular information management - DNA metabolism and gene expression.
We will emphasize What we know and How we know it. (But dont forget about Why)
20. You have preliminary data to suggest that expression of your favorite yeast gene
(YFYG) is regulated at least in part by histone H3 acetylation. You have a candidate
histone H3 acetyltransferase (HAT). You make a mutant, HATm, containing a point