Plasmid Miniprep report (marked out of 10).
Each person should write his/her own report; i.e. you should not be copying from
each other, although you can look at data together and discuss resultsbut write your own,
Topics for week 8
Exams will be returned
Week 8 Lab: Isolation of Genomic DNA and total
RNA from Drosophila melanogaster adults
come prepared: This is a BUSY
Week 12 Lab: Sequence analysis of rRNA data
set (posted on Canvas)
Learn how to analyze a sequencing
Perform sequence alignments
Big Dye terminator sequencing run: One lane or
capillary, 4 ddNTPs each covalen
Detailed Instructions Lab3Miniprep
MBB 308 Lab
Plasmid DNA miniprep, restriction digestion and gel electrophoresis
*Your first written report is for this lab see separate instructions posted
In this lab you will purify DNA from overnight bacterial culture
Mapping Problem for MBB 308
You have a 5.3 kb insert cloned into the PstI (P) site of the vector pUC19, which is 2.7 kb
in size. This vector also has recognition sites for several other restriction enzymes; all of
these sites are in the same place, adjace
MBB308 Spring Semester 2015
Practice Problem Set #1.
We will discuss solutions in Week 2 lecture and laboratories please come prepared. I
will likely ask individuals to discuss these problems in class or laboratory. You will be
quizzed on this material in
Week 3 Lecture Aims:
1. Questions from last laboratories?
2. Gel Analysis and intro to restriction enzymes
3. Common nucleic acid notations & DNA synthesis of
4. RNAP and DNA promoter binding motiv
Week 4 Lecture Aims:
Questions from last laboratories?
PCR: how does it work? Why are we doing it?
How to design good primers
Double digests with restriction enzymes