BIOL 253 Assessment for Genetics Practical 1 (week 13)
1. Plasmid Preparation. The following calculations relate to the pGEM-n11 culture.
the E.coli culture contained 2.5 x 109 cells/ml
each cell contains approx 600 copies of pGEM-n1
Glycogen- energy storage
Glycogenolysis :The breakdown of glycogen and releases glucose by the enzyme
Glycolysis: a process that provides ATP to the muscle cells as an energy source.
pyruvate: one product of glycolysis, into the Krebs cycle
Glycogen in li
201 Lec 7: The FoF1 H -ATP synthase
ATP synthase (F & F portions) catalyse H flow & ATP hydrolysis/synthesis
structures of F and F & their sign. to ATP syn.
mechanisms underlying ATP syn.: Rotational catalysis & binding change mechanis
1. -Summarise H+ transport in the mitochondria
-explain how it is mediated by a group of complexes (respiratory chain form an e -transport chain)
-Explain the sign. of H+/2e- stoichiometries and the measurement
3. - Explain in detail the H+ pumpi
-Production of Acetyl CoA
-Pyruvate is oxidised to acetyl CoA and CO2
-Occurs in the mitochond
Citric acid cycle carbon balance
-1953 Nobel prize (Hans Krebs)
the reactions of the citric acid cycle
Octet rule and covalent bonding
Covalent bonds : formed when atoms share electrons.
A general rule of thu
Light driven p+ transport
pathways for e- transfer & p+ translocation by photosystems in photosynthetic
pathways for e- transfer & p+ translocation by photosystems in plants
Compare and appreciate: the magnitude of the relevant componen
A collection of coupled and interlinked series of chemical reactions which starts with a
particular molecule and converts it to some other molecule or molecules.
1 extract biologically useful energy from the cells environment
the molecular mechanisms involved in the synthesis of adenosine triphosphate (ATP).
ATP : is essential in all cells
- it is the 1o molecule used for capturing
- transferring free energy in all biological systems.
The energy released by the ATP hydrolysis
BIOL115 Coursework 1
Determining the enzymatic properties of polyphenol oxidase
You should complete this CWA on this form and submit it as hard copy it along with a CWA cover sheet to the BLS
coursework box by the deadline of 1:30 pm, Tuesday week 6.