Lecture 3 topics
Reading nucleic acids sequences
* Restriction analysis
* Synthesis of DNA
* Role of synthetic DNA
* DNA sequencing Maxam-Gilbert, Sanger, etc
* PCR (but not real-time or digital)
Manipulating nucleic acids f
Starting with a chromosome as material to get the sequence information out of.
Do not need to know for exam!
Restriction nucleases cut the DNA fragments.
Enzymes that you find in different types of bacteria and t
Replication is bi-directional
John Cairns, 1963; Huberman and Riggs, 1968
This Week's Citation Classic
Cairns J. The bacterial chromosome and its manner of replication as seen by
autoradiography. J. Mol. Biol. 6:208-13, 1963. [Dept. Microbiology,
DNA Replication - - Feb. 6th and 13th
DNA Repair - - Feb. 27th
Prof. Grant W. Brown
Dept of Biochemistry and Donnelly Centre
Reading for todays DNA replication lecture:
THE JOURNAL OF BIOLOGICAL CHEMISTRY
2003 by The American S
BCH311 Lecture 7
February 27, 2015
Garrett. Biochemistry, 1st Canadian Edition
pp 950-959 (28.8 and 28.9)
Study task #2 is posted on Blackboard
Why DNA repair?
DNA is a rarity among biological molecules-whe