How did scientists observe cell division?
What did scientists observe?
1. Not all cells in a culture, would be dividing at the same
2. Very few cells could be observed in the process of cell division at any given time
Chapter 2: pages 51-65 and Chapter 3: pages 125-152.
Hydrophilic, usually charged; hydrophobic: usually uncharged
Ionic > hydrogen > van der Waals
Ionic/hydrogen electrostatic attraction is weakened by presence of water, less
attraction for each other,
pg. 202-206, 208-218, and 263-276.
Chromosome are long single linear strands of DNA molecule (associated with
histones/non-histone proteins) The proteins fold and compress DNA into
Homologs pair of paternal DNA, i.e. homologous DNA, o
Binding of initiator protein (YES ATP)
Unwound by helicase, Ss binding protein
prevent H-bond of DNA with itself
Primase makes RNA primer
Primosome (helicase and primase, on
DNA polymerase III
1. Grind flies and homogenize for
30 seconds till fine
2. Add 125 ul of SB+ (or SB- for
alternative) into the tube and
grind for 10 more seconds, rinse
debris off and add another 125
Part 1, restriction enzyme digestion of unknown sample
1. Label two 1.5 mL centrifuge tubes as
Unknown, and one as Master Mix
2. Create master mix from reagents
3. Add 5 uL of master mix to each of
All cells store hereditary information in the same linear chemical code DNA
All cells replicate DNA by templated polymerization
Each monomer/nucleotide in nucleotide has a base ( ATCG ) and a sugar
All cells transcribe DNA into RNA
Genes and their encoded proteins are co-linear; that is, the
order of amino acids in proteins is the same as the order of the
codons in the RNA and DNA.
In comparing the complete genomes of five bacteria, one ar
BIO130 Library Assignment Worksheet
Please attach this to the front of your printed journal pages
TA and Lab Section: _
Citation: Use the CSE style
to cite your peer-reviewed
review and primary research
Membrane Transport using
channel proteins and
tran rt r Passive Trans ort
Active Transport spo e s p
Requires carrier/transporter proteins
interactions with solute
Secorliirgofuve Primary active Imp e :
transport simple, non-polar
spread out (02,
What is a liver cell?
How much does cytosol take up in this cell, what does it do and what does it contain?
Cytosol takes up half the cell volume (not for all cells only this particular cell)
In cytosol, there is protein synthesis
Biology Part 2 Yips Section
What does the animal cell have that is specific to animals only?
- Lysosomes: the break down cell parts that are no longer needed
Extracellular matrix: specialized material found outside of the cell
What does the pl
What are going to talk about in this lesson?
Stuff between cells
Weve talked about stuff in cells so far
What are the two types of tissues?
What do epithelial tissues do?
Lines cavities, organs, and surfaces (lumen)
What is the lipid bilayer permeable to?
Can take small hydrophobic non-polar molecules (O2, CO2, N2)
Cannot take larger molecules as fast
Although if given 3 billion years, yes it will be permeable
Small uncharged polar molecules (H2O, Urea, G
What happens in the metaphase?
Forms a mitotic spindle
DNA is in middle
Mitotic spindle depolymerize
Pulling DNA to either ends of the cell
Cell divides in two
Paclitaxel prevents depolymerisation, cannot pull DNA to either end
Cell trapped in