MICRB 265: General Microbiology
Course homepage on eClass (Moodle) https:/eclass.srv.ualberta.ca/portal/index.php
Introduction and brief history of microbiology
Practice Exam Questions: Enzymes
1. The diagram shown illustrates the free energy changes in an uncatalyzed reaction. Which of
the following statements is TRUE?
A. X is the free energy of the transition state intermediate.
B. This reaction is energeticall
Lab 4: Environmental Survey Using RODAC Plates; an Investigative and an
Inquiry-Based Lab Exercise
Clean is a relative term. Disinfectants: used on inanimate surfaces to control/kill
bacteria. Non-specific in action.
Bacteriostatic: their action inhibits
Lab 8: Determining the Activity Spectrum of Antibiotics using a Modified KirbyBauer test
Kirby-Bauer disc diffusion assay:
Used to determine antibiotic sensitivity of organisms
Diffusion of a water-soluble antibiotic creates a concentration gradient. In
Next gen sequencing:
- Understand the scale of it: amount of data produced and how long it takes, compared to
- Illumina: 100-500 bp reads (shorter than Sanger but getting closer)
Up to 3 billion reads per run (compared to 100 with Sang