GAPDH Polymerase Chain Reaction
In this experiment, a portion of the glyceraldehyde-3-phosphate dehydrogenase
(GAPDH) gene will be cloned. Because it is a vital metabolic enzyme involved in
glycolysis, one of the most basic of biolog
Sequencing and Bioinformatics!
The Development of DNA Sequencing
Sequencing DNA means to determine the exact order of nucleotides (guanine (G),
adenine (A), thymine (T), and cytosine (C) in a DNA molecule. DNA
Preparation of Plant gDNA
Why Extract Genomic DNA?
Genomic DNA isolation is the first step for many downstream procedures such as
cloning, PCR, and restriction endonuclease digestions. These applications require the
isolation of high
Ligation and Transformation
Cloning is the production of multiple exact copies of a piece of DNA, usually a gene,
using molecular biology techniques. Cloning is frequently the first step of a research
project, producing enough DNA fo
Bio-Rad Laboratories, Inc.
2000 Alfred Nobel Dr.
Hercules, CA 94547 USA
Aurum Plasmid Mini Kit
For technical service, call your local Bio-Rad office, or
in the US, call 1-800-4BIORAD (1-800-424-6723)
Many laboratory exercises and experiments require one to make serial dilutions of
bacterial cultures or virus cultures in order to calculate the concentration of bacterial
cells or virus particles in the origi
The microbial culturing kit provides the materials and methods for a wide variety of
microbiology activities. The kit can be used to make both liquid and solid media suitable
for the growth of small or large cultu
Analysis of Plant gDNA Preparation
Before you can proceed to the PCR experiment, you will have to analyze and quantitate
the gDNA that you have prepared. This is to ensure that 1) you actually do have gDNA
in your sample; 2) that the
J. Dairy Sci. 91:151159
American Dairy Science Association, 2008.
Ability of Organic and Inorganic Bedding Materials to Promote Growth
of Environmental Bacteria
S. Godden,*1 R. Bey, K. Lorch, R. Farnsworth,* and P. Rapnicki*
Serial Dilution and Bradford Assay!
The Bradford protein assay is a simple and accurate procedure for determining the
concentration of protein in solution. It involves using a dye that binds to proteins. Under acidic
Biology of Cancer
Outline of the class
Introduction to cancer
Causes of cancer
Molecular biology of cancer
Discussion of research articles
Why study cancer?
1 in 5 people in the developed world will