Certain genetic diseases caused by defective mitochondrial proteins are inherited only maternally, in
contrast to most genetic diseases, in which either parent can pass on the defect. Maternally inherited
mitochondrial diseases result from muta
Macromolecules Are Synthesized and
Broken Down within the Cell
Removes equivalent of a water molecule to link
Builds macromolecules from smaller subunits
Adds the equivalent of a water
Lecture 7 Beginning Lecture on Nucleic Acids
Nucleotide General Info
-Composition: Nitrogenous base linked to sugar and at least one phosphate group
-Bases are purines (Adenine, Guanine) or pyrimidines (Cytosine, Thymine, Uracil)
-Purines bond to sugar vi
Biochemistry Lecture 15 Replication - DNA Repair
Prokaryotic Replication Cont.
-Prokaryotic DNA vs Eukaryotic: We have multiple non-specific origins of replication, prokaryotes
have one specific oriC.
-Remember helicase is on the lagging s
Lecture 16 Notes
-MutH, MutS, and MutL
-MutH is looking for a heavy methylated area of the strand.
-The appropriate polymerase then comes in to fill and ligase to ligate it. For prokaryotes its
Biochem Lecture 9 4/27/2016
-cDNA Libraries: problem is when youre transcribing, promoters are not included.
-Enhancer regions are used to activate turn on promoter so gene is able to be used.
-Enhancer allows for tight binding so, the reade
Prokaryotic DNA Replication
-Replication initiation requires helicase and primase
-Remember A and Ts at 15 kj/mol are weaker than G and Cs at 60 kj/mol
-Leading and Lagging Strands.
-Find center axis, you have two templates that
Biochem Lecture 10
Continuation of Nucleic Acid Metabolism GO OVER AGAIN WITH WILEY PLUS TO MAKE
SURE YOU KNOW THE CONCEPTS
-With activations, graph shifts to the left, any inhibitions would shift to the right.
-Know the enzymes that control the
If W is positive then you have supercoiling.
Topoisomerases, alleviates any supercoiling to access DNA to get at a template.
-Type I isomerases: makes 1 stranded cuts. ATP independent.
-Type 1A -relax negatively supercoiled DNA, make W ne
Fractionation of Nucleic Acids
-Intercalation agents lengthen the strand and increase the distance of the bonding,
making it destabilized.
-W. E. S. Blotting
-Histones to compact DNA.
-Most interactions are van der waal
Lecture 11 Nucleotide Degradation
*Realy know this*
Nucleotide nucleosides bases uric acid
-Lose nucleotidase you get a bunch of monophosphates (nucleotides), cant break down to
-deaminases can occur at different levels.
-you shut down ADA, you get