Measurement of Creatinine
Why analytical performance is
important for clinical care.
How is Creatinine Measured
Chemical reaction - Jaff reaction
Creatinine reacts with picrate (yellow compound) ion in
an alkaline solution to form a red-orange product
BIOCHEM 3H03 FINAL REVIEW
Lecture 1-Pancrease Insulin and Glucose Metbolism
-release of insulin stimulated by
Sympathetic and parasympathetic innervation of pancreas
-release inhibited by
Test Performance Characteristics
What is normal?
Is the result different from the last time this
patient was tested?
Diagnostic utility of tests. Is the result
consistent with the clinical findings?
What can the Kidney Tell Us?
1. About its own health?
2. About the health of other organs?
Can be roughly divided into
Acute Kidney Injury potentially reversible
Chronic Kidney Disease develops over many years.
The common end point of man
How do we asses the function
of the kidney?
Evaluation of Kidney Status
Examining the nephron function of glomerular
Examining the secretory capacity for particular
endogenous and exogenous compounds.
Evaluating the kidneys reabsorptive capa
Some Illustrative Cases
Examples of fluid and electrolyte cases
How do we measure all the tings we have
been talking about ?
Which of the following two situations results in
a greater reduction of extracellular fluid
Dept. of Pathology & Molecular Medicine
905-521- 2100 x 76595
Dr. S. Hill, 521-2100, Ext. 76595
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What does the kidney do?
How do we assess kidney function?
What can the kidney tell us about other
What do the kidneys do?
Regulate body fluid osmolality and volume
Regulate acid-base balance
H+ and Bicarbonate (HCO3-), se
Intrepreting Acid:Base and
Some Case examples
Acetylsalicylate (ASA, Aspirin) metabolized to
Common analgesic, anti-inflammatory, antiplatelet
Used in prevention of coronary artery disease
Acid:Base Metabolism and Body Buffer
Chapter 3 in Marshall is Important
Hydrogen ion - H+
[H+] in plasma 35 45 nmol/L (note units)
pH 7.35 7.45
Bicarbonate ion HCO3
[HCO3] 22 26 mmol/L
Dissolved CO2 we usually me
BIOCHEM 3H03 MIDTERM 1 REVIEW
Lecture 2- Test Characteristics
-repeated measure of a single sample will give a normal distribution of values
-mean +/- 2 SD is considered normal
-however, abnormal result does not mean disease pathology and a normal result