Dr Jo Hobbs
Biochem 300A - Guest lecture
1. Sequence structure function
2. Identifying homology and aligning sequences
3. Sequence identity vs. similarity
4. Divergent and convergent protein evolution
chapter 10 in textbook
topic 2 (Ch.10) assigned reading
introduction to enzyme regulatory strategies
l i and
BIOC 300A Fall 2015
Practice Exam 2
INSTRUCTOR: Dr. D. Briant
COMPLETE THIS EXAM AT HOME.
WEDNESDAYS LECTURE (NOV. 18) WILL BE DEDICATED TO REVIEWING THE
30 MARKS TOTAL
Economy class syndrome was a name given to a condition where people on
Suppose that a turn of B-DNA in a circular DNA molecule with L = 100 and W = -4 becomes a turn of ZDNA .
a) What are the L, T, and W following the transition?
b) How does this transition alter the electrophoretic mobility of this DNA in an agarose gel?
1. Free energy and spontaneity
2. The role of catalysts
3. Understand HOW enzymes increase rate of
4. Binding energy
Equil reects G of
Rate depends on
chapter 12 in textbook
topic 4 (Ch.12) assigned reading
you will need to read the entire chapter for this section,
except the section on lipid rafts (pg 358) and eu
Enzyme catalyzed rxn begins with
binding of substrate
Lower acCvaCon energy
Establishes substrate specicity
AcCve site incorporates a highly reacCve
topic 5 (Ch.13) assigned reading
introduction to membrane channels and pumps
families of ATP hydrolyzing pumps
UNIVERSITY OF VICTORIA
Dr. MJ Boulanger
120 mins (approx.)
TO BE ANSWERED IN THE SPACE PROVIDED. COUNT THE NUMBER OF PAGES IN
THIS EXAMINATION PAPER BEFORE BEGINNING TO WRITE
Briefly describe the major steps involved in DNA isolation.
Provide a brief classification of the main types of eukaryotic RNA polymerases,
Indicating the type, location in the cell and transcripts synthesized.