Molecular Biology of the Cell
Department of Molecular and Cellular Biology
University of Guelph
This course builds on the fundamental concepts of genetics and molecular biology that are covered
in MBG*2040, and conti
23. The following sequence is the sense strand of genomic DNA of the Huntingtin gene. The
CAG repeats are in lower case and in brackets. The underlined region is used to design 6~
nucleotide PCR- primers to amplify the CAG repeat region for diagnosis.
PART A (Choose only one and the best answer among the choices given.) I
1. After cutting DNA, which of the following enzymes is used to join the ends of two DNA
a) restriction endonucleases
/" b) RNA polymerase
V 0) DNA gyrase
@ DNA ligase
(1 mark for each COMPLETELY correct primer, both the sequence and DNA polarity).
5 AAGTCC 3
5 GTCTGG 3
Normal alleles have 10 to 29 CAG repeats 30 to 87 bp in length of the repeat tr
PART A (Choose only one and the best answer among the choices given.)
\ 1. Which of the following enzymes is required in a Sangers DNA sequencing reaction?
a3 _.erym , e
More than one of the above
2. After cutti
Molecular Biology of the Cell *2050
MCB Study Notes- FOR FINAL
eukaryotes have a membrane bound nucleus with a nuclear envelope, nuclear pores and an
internal nuclear structure
prokaryotes have a region where the chromosome is located (nucleoid)
Operons are common in bacteria but not in eukaryotes. Suggest a reason why.
ANS: In multicellular eukaryotes, the environment of an individual cell is relatively
stable. There is no need to respond quickly to changes in the external enviro