Lecture 1: Central Dogma theory of DNA: 10/09/2009 Crick's idea was that genetic information was stored in DNA which is transcribed into RNA and then translated into protein which performs activity in the cell. mRNA was not discovered yet, but they alread
Recombinant DNA technology!
practical use of cells and DNA!
Molecular Cloning: !
insertion of DNA fragment from one cell/
organism to another type of cell!
Berg, Cohen and Boyer!
mutant strains of Esherichia coli lacking
building blocks of proteins
contains (at least) 1 carboxyl
and amino group
Figure 5.3 General Structure
of the -Amino Acids
a) D and L (aa are L)"
b) R and S system"
Amino acid vary in:"
BIOL 2020 3.0!
Dr. Terry Kubiseski!
Tues/Thurs 12:30 1:30 pm!
Rm 213 LSB!
I will try to respond within one working
PLEASE include a brief and descriptive
subject line in your emai
- process in how cells acquire,
transform, store and use energy!
coordination, regulation and energy
the energy input is in the form of fat
and carbohydrates or!
light is the source of energy!
Protein Synthesis : Translation!
transfer of genetic information into
protein amino acid sequence!
Occurs on Ribosomal Particles!
Function of Ribosomes!
move along mRNA template and convert
genetic code to amino acids!
Structure of DNA and RNA!
Structural Characteristics of
nucleic acids have acidic region!
Variable region : hydrophobic (aromatic), not
protonated and uncharged!
First midterm, October 8th
Surname: A to O Room LS A
Surname: P to Z Room LS B
KM = [S]"
vo = 1/2 Vmax"
Plot of 1/vo vs 1/
Useful when comparing different
substrates for the same enzyme.
lue, and so
monosaccharides / polysaccharides!
role in metabolism!
perform structural functions!
component of DNA, RNA!
covalently linked to proteins and lipids!
Formula for : (CH2O)n where n is 3 to 7!
one aldehyde or keto
DNA: replication and repair!
Replication: duplication of genetic
!Prokaryotic Replication easier to
Two possible models for DNA replication!
Meselson and Stahl!
Grew E.coli in NH4Cl with (15N)!
Transferred some of th
Structure of Antibodies!
-involved in immune response, also called
-five classes of antibodies (IgG, IgA, IgM, IgD
-binds molecules considered foreign!
-Consists of 4-polypeptide chains linked
together through disulphide b