Enzyme Dynamics and Function
- Last week was all about enzyme kinetics and regulation.
ES E + P
A Combined Experimental and
Computational Strategy to Define
Protein Interaction Networks for
Peptide Recognition Modules
Amy Hin Yan Tong." Becky Drees. Giuliano Hardelli.
Gary D. Bar:ler."'"I Barbara Brannetti.3 tuisa Castagnolif
Siueliftum-Hm: L, We Ogiwalcl DIWHQHIQIJ
- iiolde wuibnan afone 'mtdn'l' a1 a time
5y Hing le contenhIm at" all but one
adult in false (55 and So am dint
u for - 37: 1. mm
H [81in huge was , [g-MSW 9; [5'].
ml .4 2: ABLE = 4.:sz
M is g Eseudaimi
Distinguishing between cooperative and unimodal
downhill protein folding
Fang Huang*, Satoshi Sato*, Timothy D. Sharpe*, Liming Ying, and Alan R. Fersht*
*Medical Research Council Centre for Protein Engineering, Hills Road, Cambridge CB2 2QH, United Kingd
Biochemistry 2001, 40, 6180-6189
pH-Induced Conformational Transition in the Soluble CuA Domain of Paracoccus
denitrificans Cytochrome Oxidase
Sayan Gupta, Antony Warne, Matti Saraste, and Shyamalava Mazumdar*,
Department of Chemical Sciences, Tata I
- Mostly, we think of nucleotides as being part of RNA/DNA, but
now that weve studied metabolism, we know theyre all over!
So how do we make these things?
Amino Acid Metabolism
Most of the Animal Kingdom = Lazy
- Most higher organisms in the animal kindom dont bother to
make all of the amino acids.
- Instead, we eat things that make the essential amino acids for us
Unsuprisingly, humans are among
Welcome to 4081: Principles and
Applications of Mass Spectrometry
Course Director: Derek J. Wilson
Email: [email protected]
Office Hours: After Lecture, CB318
Week 2: Soft Ionization
Mass Spectrometry is super-cool:
A Brief History of MS:
So far, the ionization techniques weve talked about are:
At least somewhat hard; they tend to make ions through
Enzyme Regulation and Mechanisms
Enzymes in vitro
- Ok, so we know a few tricks that enzymes use to catalyze
reactions by stabilizing the transition state.
- But how do we uncover these mechanisms?
- How do we figure out their fundamental proper
Enzymes and Protein Structure
We (Re)Learned About
And Tertiary Structure
What are these crazy things called Enzymes?
Why do we care about them?
- Enzymes a
Welcome to Biochem 3050 / Biology
3010: Enzymes and Metabolism
Course Director: Derek J. Wilson
Metabolism What is it?
Websters the sum of the processes in the buildup and destruction of protoplasm;
specifically : the chemical changes in living cells by w
- In Glycolysis and the citric acid cycle,
weve made a lot of reduced cofactors
NADH and FADH2
- In oxidative phosphorylation, we use the
energy generated by reoxidation of these
cofactors to make ATP
Metabolic Pathways and Energy Metabolism
- The first thing a living organism has got to be able to do is
harness energy from the environment
- Plants do it by absorbing sunlight
- We do it by eating food
- I suppose this explai
Amino Acids to Peptides to Proteins
- We learned all about Pee!
- And Metabolic Pathways
- And Enzymes
- And the Organic Reactions that Keep us Alive
1780 - 1855
Karl Ritthausen 1866
Table 25.3 Integrated rat laws
Order Reaction Hate Iaw r5
[I Ar F' V a 1k 2k
1 heP u=mm .%
H = In
P m]! L
E lll U Mn
RHINO - I!
+ Ba? _ v = HARE]
1 ln [Mullah - x]
[Bln - IND Mn - 1 EL
A+2Et>F' v = HAIR]
1 m [AHEB
SDBS-1H NMRSDBS No. 10602HSP-02-281
C5 H8 O2
0.04 ml : 0.5 ml CDCl3
21 t 16
.5 o .3
SDBS No. 10602HSP-02-281
(c) National Institute of Advanced Industrial Science and Technolog
SDBS-1H NMRSDBS No. 10016HSP-00-346
C6 H4 Cl2 O
0.052 g : 0.5 ml CDCl3
SDBS No. 10016HSP-00-346
(c) National Institute of Advanced Industrial Science and Technology (AIS