Biochem Oct 4
SDS and western blots how they work and what they are for.
SDS page gels slab or potentially of acyrmilide organic polymer. You prospect it
through polymerization rxn and goes through a gel (put in the wells inside, put a sam
1. What enzyme(s) is (are) responsible for the following reaction?
Pyruvate + CoA + NAD+ acetyl CoA + NADH + H+ + CO2
acetyl CoA synthetase
pyruvate dehydrogenase complex
2. Which of the follo
1. What is a cytochrome?
a protein that transfers electrons, and that also contains a heme prosthetic group
a chloroplast protein that transfers electrons, and that also contains an iron sulfur
a protein that pumps ATP, and that
Choose the correct answer from the list below. Not all of the answers will be used.
a) initiation facors
f) elongation factor Tu
j) release factors
Which of the following elements is most
abundant (in % atoms) in the human body?
Both H and C are
Which carbon atom is part of the
What is the pH of a
Week 1 questions
1. A patient with poorly controlled Type I diabetes has blood drawn and finds
that the pH of his blood is 7.1. The normal pH of blood is 7.4. The patient's
blood [H+] is
one fourth normal
one third normal
1. The difference in RNA bases compared to DNA bases is
RNA contains A instead of T.
RNA contains A instead of U.
RNA contains U instead of G.
None of the above.
RNA contains U instead of T.
2. How many different sequence possibilities are
1. An aldehyde and alcohol can react to form a
A 1,4-glycosidic bond
A 1,6-glycosidic bond
2. To which amino acid residues in glycoproteins are the sugars commonly linked?
tyrosine and asparagine
5. Use the redox potential for each of the two half reactions for lactate -> pyruvate; 0.19 (V)
1. RNA polymerase requires _ for initiation.
none of the above.
2. _ is the protein which binds promoter sequences and help RNA polymerases initiate
3. A chemica
Week 2 Questions
1. If a polypeptide has 400 amino acid residues, its approximate molecular
weight is (in units of KDa):
2. Which one of the following statements is correct?
the -helix can be composed of mo
Week 4 Questions
1. Which of the following fatty acids has the lowest melting temperature.
a. cis, cis 9,12 Octadecadienoate (18:2)
b. cis 9 Octadecadienoate (18:1)
c. n- Docosanoate (22:0)
d. n-Hexadecenoate (16:0)
e. trans, 9 Octadecadienoate (18:1)
BIOCHEMISTRY October 18, 2012
CHAPTER 17 Preparation for the cycle
Pyruvate to acetyl coa is a permantant change, and that change is going to promote the
electrons from the acyl group, and allow energy to be made as well enters the citric acid
Biochemistry Oct 11
Chapter 9: carbohydrates, Chapter 13, Chapter 14
Carbs essentially are sugars, organic molecules. Ketones or aldehyde with alcohol, and
they are reactive.
Monosac aldehydes or ketones that have two or more hydroxyl grou
Biochem Oct 2
CHAPTER 12 CHAP 41 (antibodies not tested on, he may not even go over it)
EXAM: M/C about 25
Fill in the blank: 10 (or 9) key words or formulas. Identify the elements of a particular pathway
or a molecule (spelling, not so worried about)
Biochemistry September 11, 2012
CHAPTERS 2 &3: Buffers and Amino Acids
Which of the following elements is most abundant (in % atoms) in the human body?
Which carbon atoms is part of the carboxyl group?
Buffer is a weak acid or
Biochemistry Sept 20
Chapter 5&6 basic concepts of enzyme action kinetics & regulation
Enzyme is essentially a protein. Ex/ glow of the luminescent jellyfish
Another enzyme carbonic anhydrase: faster enzyme, 10^6 molecules of CO2 per
Biochemistry sept 25
CHAPTER 7: Mechanisms and Inhibitors
Covalent cat active site contains an active group (some sort of nucleiophile), and
temporarily covalently modified in the course of catalysis.
Generalacid molecule other then wat
Biochemistry September 18, 2012
Chapters 4 & 8
3D shapes and how it folds.
Hyrophobic residues in the interior and polar outside (this makes it soluable)
Yellow hydrophobic (inside)
Within the cell, it acts as an oxygen buffer
Heme, a prosth
Biochemistry September 13
Chapter 4: Protein Three-Dimensional Structure
Levels of pr
4 levels of protein structure: 1st level primary structure amino acid sequence
Secondary structure formed of a repeating unit of amino acids 2D structure - 2 types,
Week 3 Questions:
1. Reaction coordinate diagram for uncatalyzed and enzyme-catalyzed
conversion of a reactant S to its product P (S<->P): Activation energy for the
catalyzed conversion of S->P is represented by the arrow:
2. If t
Biochemistry September 6
CHAPTER 1&2: Biochemistry and the unity of life water & weak bonds
Living systems require a limited number of atoms & molecules
We are mainly made up of hydrogen, oxygen and carbon
Carbon is an essential element, it serves as a