Proteins Why is the majer greeve at DNA informationrich?
male: greave majnr grunve
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Changes to DNA which dont involve changes to nucleotide sequence. I.e. changes to histones
Control of DNA accessibility
Regulation of gene expression
DNA editing and repair
Structure determines function. Structure allows us to identify a protein.
Misformation of structure changes protein
Level of protein:
Primary: Linear sequence of amino acids
Secondary: Regular repeating interactions between nearby reisudes, mainly inv
Different species have difference genomes, but certain characteristics allow us to perform techniques for
1 allele from mom, 1 from dad
Genomes vary widely in size from species to species
Genomes vary widely in information content
DNA molecule composed to two anti-parallel strands, where deoxyribose nucleotides are held together
covalently by phosphodiester linkages.
A-T (2 hydrogen bonds)
G-T (3 H bonds) There this is a stronger than A-T
This bonding is cr
pH important for enzyme function
Blood pH 7.35-7.45 -> Hb works best in this range to allow respiration to happen. If pH changes in either
direction, acidosis and alkylosis can occur.
pH = measure in hydrogen ion concentration. Expressed as logarithmi
Structural elements of the E.coli replication origin
DNA sequences of known origins share 2 common features
DnaA 9-mer site - 9 nucleotide recognition site. They have directionality to them
Repeating sequence recognized by DNA binding proteins (DNA A
BCHM 218 Week 1
Cells store DNA, information must be expressed for cell to carry out function
DNA -> RNA = Transcription
RNA -> Protein =Translation
DNA information encoding molecule
Crick proposed that biological information flows in this direction (cent
Eukaryotic cell cycle (4 stages) Copy genetic info and transfer to next generation
Every time a cell divides it replicates all of its DNA; a process that takes place in a matter of hours
Eukaryote genomes are large nearly 3 billion bases in the human
What is pH?
' pH which is a measure of hyd regen inn cnncentratien
Why is it important at understand pH?
' pH is critical tn many impnrtant chemical reactiens,
the bicrchemistry crf melecules, and life.
Why is pH signicant?
' pH influences the structure a
Which of the following is true of ligation reactions?
They can occur between any fragments having sticky ends.
They only occur between fragments having blunt ends.
They can occur between fragments having complementary
sticky ends or between