The figure below represents the restriction map (using EcoR1) of the gene DET 2 from Arabidopsis thaliana.
The distance between the cut sites is presented on the figure. Describe the results of a Southern blot
experiment performed with DNA samples coming
Name_ ID _
1- (5 points) The E. coli tryprophan operon is subject to regulation by attenuation (10-fold)
and repression (70-fold). The lacZ reporter gene shown in the figure below has been
The gene det2 was clone in the EcoR1 site of pBluescript.
Using the map presented below, design a simple experiment that will allow you to find out if the gene is
inserted in the proper orientation. .
Biol 367 Molecular biology
ID number: _
1-(3 points) On the same graph, show the expected Cot curves and the Cot 1/2 values
for the bacteriophageT3 genome (30 X 10 3 bp) and the non-repetitive portion of the
mouse genome (Note:
2. A linear DNA molecule is labelled at both ends with P32 and digested with
restriction enzymes. HindIII produces fragments of sizes 2.9, 4.5, 6.2, 7.4 and 8.0
kb. An autoradiogram developed from a Southern blot of this digest shows
1. The following mutations (mutant bases are shown in small letters) occur in the leader
polypeptide gene region of the tryptophan operon. Also shown is the wild type sequence of the
coding strand of the leader peptide gene.
Note: (i) Assume that there is
2. Describe the fate of the following mutant lambda phages after they infect E. coli. Explain your rational.
mutant #1- inactive CI gene.
mutant #2- inactive cro gene.
mutant #3- inactive Prm promoter.
mutant #4- mutant causing a defective att site.
6) (5 points) Consider both aspects of control (negtive and positive) of the lac operon. How much [3
galactosidase activity would you expect each of the following strains to express under the growth conditions
Note: cap = cAlViP binding protein
-First allosteric enzyme discovered by the scientists Carl and Gerty Cori.
-Responsible for the catabolism of glycogen (phosphorolysis) in the liver, brain and muscle
-Tetramer, but acts as 2 homodimers
* We will stu
1) You have in your lab a genomic DNA fragment with five primers (1 to 5). The position of annealing of
teach primer on the DNA fragment is illustrated below.
Three PCR reactions (A,B and C) were performed u
RNA processing capping and
almost all transcripts are processed by intron removal capping
Eukaryotic mRNA are altered post transcriptionally
at their 5 ends
-5 end modification involve
Chapter 8: Major Shifts in Bacterial Transcription
Chapter 8 Opener
How long in m is the 168,903-bpT4 genome?
Estimate the number of protein coding genes in the T4 genome.
Major changes in gene expression caused by changing
November 3, 2000
1) You characterized four E. coli mutant strains in the lac operon (M1 to M4) by Southern blot and you
obtain the following results (WT = wildtype).
Note: The structure of the lac operon and the restrict