Metabolism: Basic Concepts
Chapter 15: Outline
15.1 Metabolism is composed of many coupled, interconnecting
15.2 The oxidation of carbon fuels is an important source of
15.3 Metabolic pathways contain many recurring moti
Estrogen receptor transcription and transactivation
Structurefunction relationship in DNA- and ligand-binding
domains of estrogen receptors
Marc Ruff, Monique Gangloff, Jean Marie Wurtz and Dino Mora
ZymoPURE Plasmid Maxiprep Kit
Catalog Nos. D4202 & D4203 (Patent Pending)
The fastest spin-column based procedure for purifying up to 1.2 mg of ultra-pure endotoxin-free
transfection-grade plasmid DNA.
Innovative ZymoPURE bin
Presented by: Aimee Steinwand & Kaitlyn Console
Background of LDH
Role in anaerobic glucose
metabolism & synthesis.
Oxidizes lactate to pyruvate
with NAD+ OR pyruvate
to lactate with NADH
LDH is found in all livi
26 October 2016
Results So Far
We started with Lab 5 which was the first step of the enzyme lactate dehydrogenase
purification. The purpose of this lab was to begin purifying LDH by putting a cow heart in
Preparing SDS-PAGE Gels
(1) Acrylamide is a neurotoxin. You must wear gloves at all times when handling it or any un-polymerized
solution containing it. Avoid inhalation and direct contact with it.
14 September 2016
Lactate dehydrogenase abbreviated as LDH is defined in our textbook as an enzyme that
catalyzes a step in anaerobic glucose metabolism and glucose synthesis (Berg 292-3).
Lab protocol: Inoculating liquid bacterial cultures for maxiprep
January 23, 2017
*The entire procedure will happen in room 202.
1. Put on gloves. Then wipe the bench next to the shaker with 1% bleach, ethanol,
or a chlorox wipe. The shaker is on the left
8 September 2016
Now that we have successfully synthesized these 3 novel compounds, the most important
part is determining which is the most effective local anesthetic. Based on the knowledge we