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Does not require oxygen.
Can grow with or without
it. Able to detoxify toxic
by products of oxygen.
Growth throughout the test
tube. Due to ability to grow
Enteric plates (EMB & HEKTOEN)
Selective media for isolation of gram negative bacteria.
Clinical sample is stool as they are enteric (gut) bacteria, which are members of
the family Enterobacteriaceae.
Non pathogenic bacteria (coliforms): E.coli a
Cell wall is made of chitin.
Macroscopic fungi: like mushrooms and puff balls.
Microscopic fungi: like molds and yeasts.
Yeasts: unicellular, round to oval shape, depend on asexual reproduction (budding).
Molds: filamentous lik
Epidemiology is the science which studies the causes, occurrence, transmission, distribution and
prevention of diseases in any particular population.
Modes of transmission of infectious diseases:
Direct contact wit
Aseptic transfer and streak plate methods of isolation
Transfer of microbes should be done aseptically.
Avoid contamination of the culture, the medium and the surroundings.
Everything should be ready and organized like tape and marker.
Use tube ra
Multiple test system which identifies the enteric bacteria.
12 individual chambers:
Glucose, Lysine, Ornithine, Indole, Adonitol, Lactose, Arabinose, Sorbitol Voges-Proskaver, Dulcitol, Urea
Remove the cap from one side and touch t
Fluid Thioglycollate medium:
Used for aerobic and anaerobic organisms.
The media contains: nutrients like yeast extract
Thioglycollate which reduces O2 to H2O.
Resazurin which is an indicator turns pink in presence of O2 and stays colorless in O
Acid- fast stain
Organism which have mycolic acid in their walls
Mycolic acid is a waxy material which has higher affinity for the primary stain and resistant to
decolorization by alcohol.
1- Kinyoun(k): uses cold stain
Serum: add to it:
Big parasites , which some clinical specimens may contain evidence of helminth infection.
Clonorchis sinesis :
Clonorchiasis affecting liver.
Far East (J
Stains are made of solvent (ethanol or H2O) and colored molecule (chromogen).
Chromogen is the portion which gives color.
Auxochrome is the charged part of the chromogen.
Most bacterial cell membranes are negatively charged, so they can bind
1. Which kingdom consists of eukaryotes that nourish themselves mainly by decomposing organic
2. Carolus Linnaeus
D. Standardized binomial system of nomenclature.
3. The scope of microbiology include the following except
Simple & Gram Staining
MCB 3020L Tuesdays 3:00-5:50 PM
The purpose of this experiment is to perform quadrant streak-plate inoculation, a
simple stain and gram stain in order to identify the name of an unknown bacteria
Effects of UV Light on Bacteria & the Importance of Antiseptics, Disinfectants, and
MCB 3020L (T 3:00-5:50 PM)
The purpose of this experiment was to observe the effects of ultraviolet light exposure on
Bacterial DNA of Polymerase Chain Reaction
MCB 3020L T. 3:00-5:50 PM
July 19, 2015
The purpose of this experiment is to identify whether S. aureus or S.
epidermidis are growing on our skin.
PCR (Polymerase Chain Re
Exercise 3-5 Simple Stains (pgs. 95-102)
Exercise 3-8 The Acid Fast Stain (pgs.
Exercise 3-9 The Capsule Stain (pgs.
Exercise 3-10 The Endospore Stain (pgs. 117-120)
Read the lab material prior to attending this week's session. Below is
Course: MCB2010- Microbiology
Study test questions for Exam #1
1. What are bacteria?
Bacteria are prokaryotic and unicellular organisms. Bacteria have a simple organization; they
contain an external cell wall, a plasma membrane, circular DNA within the cy
C. more harmful than beneficial
D. more beneficial than harmful
E. both beneficial and harmful
Do you think you are infected if these microbiota are found on your tongue?
No, you are not infected microbe or
1. Bacteria belong to the kingdom of
2. Which one of the following statements is not part of the Koch's postulate
d. The microorganism must be isolated from a dead animal and pure cultured.
3. Which of the following is NOT a domain in the
Chapter 1 - Discussion Question
1. What are the 4 microorganisms you seen in this clip?
Bacteria, fungi, protozoa, nematodes.
2. How fungi differs from other microorganisms?
Clear cell walls and cross walls.
3. What are protozoa doing in this clip?