Denaturing electrophoresis with SDS.
SDS is sodium dodecyl sulfate or sodium lauryl sulfate, an anionic detergent that is negatively charged.
Proteins bind SDS 1.4 g / g protein, equivalent to SDS every 2 amino acids.
Before electrophoresis the protein is
Chromatographic methods or techniques:
Chromatography involves passing a solution through a medium having a relative adsorption for the
various solutes. It can develop in paper, thin layer or column. The first two are not widely used today,
thus we see th
Chemical elements in living matter
Substance consisting of combination of chemicals.
Of the 92 chemical elements 30 naturally occurring essential to living organisms.
ATOM = smallest particle of an element that has all the features of this chemical
Cellulose acetates: they had the same drawbacks, although they were more resistant.
Gels: less paper diffusion and more resistant to treatment.
Starch gels: its major drawback is the inability to determine the pore size.
They allow many tr
They are a very large group of amphipathic molecules, so that their structure and form spontaneously in
solution biological membranes. They never have backup function, but structural, and in the cell have a
complex distribution and compart
Constant speed speed
In equilibrium, "Fzas = 0 and therefore Fc - Fflot - Ff = 0.
? m r - Vp? ? r - f = 0
we put the dough in terms of molecular weight: m = M / N where
N = number of avodgrado = 0.6022 10
M = molecular mass.
Vp =? M =? M
Dialysis and ultracentrifugation techniques
Dialysis and ultracentrifugation serve to concentrate and purify biopolymers. They are based on the use
of semipermeable membranes which allow passage of small molecules but not proteins or other
One of the most important features of living things is irritability, which is that they are capable of
reacting to stimuli of internal and external environment and develop responses. The answers may be
simple or complex. For these responses o
Determination of oxidic link
This is to find out, in a monosaccharide, which atoms are involved in oxidic link. For this, two main
methods are employed.
The first is treatment with periodic acid monosaccharide, breaking CC bonds of the chains of
Concept, classification and biological importance
Carbohydrates, purely are aldehydes or ketones with many hydroxyl groups (polyhydroxy or
polihidroxiacetonas). Make up most of the organic matter in the soil for its various fu