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vThe Ionization of Water
the dissociation of water can be simplied to:
H20 .2 H+ + 0H m? Qvuwgolun.
and may be written as:
[H+] [OH-1 where KI is the equillhrium constant for the reaction.
Keq _ The concentration of w
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non-ionized form of amino acid fully ionized form of amino acid
The amino and wrboxyl group can be ionixed depending on the surrounding pH. does not ulst in appreciable amounts -major form at physio pH (6.8-7.2)
At the pH typical of cytoplasm (pH 6.8 7
Practice Questions (Solutions will be posted on D2L).
Qith is the concentlation of OH in an aqueous solution ifthe H' concentration
is 1 x 104M? Is this solution acidic or basic?
@What is the [H] in an aqueous solution of 0.2 M HCI. What is the pH?
" A Solution that resists changes in pH is said to be buffered
A buffered solution (an resist pH changes because an equilibrium exists between the
weak acid and its conjugate base.
Consider a buffer composed of the weak acid acetic acid (CH3COOH)
Using the HendersonHasselbalch equation to formulate buffer Practice Questions (Solutions will be posted on D2L).
Acetic acid has a pKa of 4.8. How many ml of 0.1 M acetic acid and 0.1 M Na acetate are 5, you are studying a enzyme in the laboratory that
Gene Expression | - Transgrigtion
. Transcription is the synthuls of RNA from a DNA template
- Flrststepin gene expression
. Only mRNA gets translated to protein molecules
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