Genomic DNA Isolation
To learn the importance of genomic DNA.
To learn the differences of chromosomal DNA and extra-chromosomal DNA.
To be able to isolate genomic DNA from Gram-negative bacteria.
Until the early 1970s, DNA was the mos
DNA Scissors: Introduction to Restriction Enzymes
At the end of this activity, students should be able to
1. Describe a typical restriction site as a 4- or 6-base- pair palindrome;
2. Describe what a restriction enzyme does (recognize and cut a
Competent Cell Preparation
At the end of this lab session, students should;
Learn how to treat bacteria to take up plasmid DNA.
Understand the process of transformation.
Know how we select for bacteria that have taken up a plasmid.
Laboratory Safety Guidelines
Biology lab safety rules are guidelines designed to help keep you safe when experimenting.
Some equipment and chemicals in a biology laboratory can cause serious harm. It is always
wise to follow all lab safety rules. Don't fo
Polymerase Chain Reaction (PCR)
To learn how PCR works.
To be able to use PCR to amplify specific DNA fragments from a template.
To learn how to design PCR primers.
Many genetic and molecular biology experiments require large quantiti
To learn what transformation is.
To learn how artificial transformation of bacteria takes place.
To be able to transform gram negative bacteria E. coli with circular plasmid.
Transformation is the process that