MCB 450 Problem Set 2 1. From the following data on an enzymic reaction, determine (a) the type of inhibition, (b) KM for the substrate, (c) Vmax, and (d) Ki, assuming that the inhibitor is present at a final concentration of 5mM in the reaction mixture.
MCB 450 Problem Set 2 - Answers 1. From the following data on an enzymic reaction, determine (a) the type of inhibition, (b) KM for the substrate, (c) Vmax, and (d) Ki, assuming that the inhibitor is present at a final concentration of 5mM in the reaction
MCB 450 Problem Set 1 1. If the internal pH of a muscle cell is 6.8, what is the [HPO42-]/[H2PO4-] ratio? (pK2 = 7.2) 2. What is the [H2PO4-]/H3PO4 ratio at pH 3.0? At pH 2.0? (pK1 = 2.15)
3. The free energies of hydrolysis for several compounds are given
MCB 450 Examination 1 Key Spring 2010 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 14. 15. 16. 17. 18. 19. 20. 21. 22. 23. 24. 25. 26. 27. E E C D A B A C E C E D C E B E B E C B A C Either 15.8 a) -9.5 kJ/mol, b) Cells might not have an enzyme cata
25. (4) Glutamine in its fully protonated form is placed into solution and titrated to its isoelectric point with NaOH (a) How many moles of base is(are) required for this titration starting with one mole of glutamine?. 25. a) 1; b) carboxyl 27.
Spring 2010 form B 7. Which of the following is an INCORRECT statement about NAD+ and FAD? A. NAD+ transfers one hydrogen as a hydride ion and the other as a proton, whereas FAD accepts two hydrogens as free radicals. B. Only one is derived from a water-s
MCB 450 Problem Set 3 Answers 1. This gives pyruvate labeled in its methyl group. Newly-synthesized glycogen would have label in carbons 1 and 6 of its glucosyl groups. 2. Glucose labeled in carbon 2 would give pyruvate labeled in its middle carbon atom,