MCB 481 Exam I Review Material 2/14/12
Know the steps of the formation of the nervous system (possibly short answer)
o Formation of neural plate
o Formation of neural grove
o Closure of neural grove to make neural tube
Neural plate markers SOX gene that
Student: Joseph Peng
Article Title: Notch and EGFR Pathway Interaction Regulates Neural Stem Cell Number
<Aguirre, Adan, Rubio, Maria E., and Gallo, Vittorio. "Notch and EGFR Pathway
Interaction Regulates Neural S
1. Prepare a written document with the following information:
1. Hypothesis (1-3 sentences)
2. Rationale for proposed studies (2-3 paragraphs)
3. Describe each figure, including methods data
MCB 481 Lecture 15 3/15/2012
you can refine the post synaptic moment.
Synapse elimination monitored by electrophysiology
o To determine if the target is truly refined, they measure the activity of the target.
o Different response for each afferent.
MCB 481 Lecture 13 3/8/12
The target and projection neuron both takes up unique characteristics when interacting
with each other.
Neurotransmitter is used to communicate between postsynaptic cell and presynaptic cell.
The space inbetween post/presynapt
MCB 481 Lecture 12 3/1/12
Axon guidance and target selection a systems approach
o Commisural interneurons
o Somatosensory cortex
o Retinal ganglia cells receive signals, then connect to the superio
MCB 481 Lecture 11 2/28/12
Mechanisms of axon guidance
o Contact-mediated attraction
Telling axon its the wrong direction
It will change axons direction
o Contact-mediated repulsio
MCB 481 Lecture 14 2/12/2012
From blastocyst to glia
Different peaks of genesis for neurons, astrocytes, and oligodendrocytes.
o Neurogenesis peaks early in development
Finish generating before the animal is born.
o Astrocytes peaks during the second p
MCB 481 Exam II Review
Glial cell formation
Know the signals
Order of development of the cell. Neurons vs astrocytes vs
Need to think about neural crest development
o Neural plate in the epidermis
o Induced rostral t
MCB 481 Lecture 1 1/16/2012
Expect to know the structures of neurons and glia.
o Soma (Cell body)
o Dentrites that receives information from other neurons
o Axons transfers electrical impulses
o Synpases at the end of axons.
o Myelin sheath protects Axon
MCB 481 Lecture 14 2/23/12
o Pax 3/7
Critical downstream targets for signaling molecules.
o Once a neural crest, nothing can alter its fate.
Have to migr
MCB 481 Lecture 8 2/9/12
Dlx1 is needed for GABAergic neurons.
o Turned on down stream of pro neuro gene.
Homeobox transcription factors specify regional identity.
o Pax6 and Emx2
Important for cortex.
Pax6 and Emx2 participate in cortical neurogenesi
MCB 481 Lecture 7 2/7/12
o Anolog gets incorporated into cells.
o If injected early in development, the marker will get diluted.
Because cells are very actively dividing.
If injected when the cell no longer divides, the signa
MCB 481 Lecture 6 2/2/12
Becoming a neuron
o Stem or founder cells turn into multipotential progenitor
o The Multipotential progenitor can then turn into neurons or glial cells.
o Neural precursor vs glial progenitors.
o VZ = ventricular zone
MCB 481 Lecture 5 1/31/12
o Retinoic acid signaling dictates hinbrain boundaries.
o Hox genes specify regional identity
o Shh = sonic hedge hog is important to get motor neuron to form in the first place.
o The roof plate that has
MCB 481 Lecture 4 1/26/12
Xenopus cells can be used experimentally both in vivo or in vitro.
Easiest way to do a gain of function experiment by havng expression vector
o Using plasmid.
o Open up the plasmid and put in the gene in question and see how it
MCB 481 Lecture 3 1/24/12
Now that we have a neural plate, what do we do with it?
o Generate structures and cells with distinct functions
o Pattern anterior.posterior & dorsal/ventral
By start patterning, we no longer have just generic nerual tube, but
MCB 481 Lecture 2 1/19/12
When does neural induction begin and is it the same in all species?
o Animal cap is going to be formed by ectoderm.
If the animal cap is removed during pregastrula stage, the animal cap turns
If the animal cap
Student presenting article
Presentation: Start _
Rate the speaker on the following (1=Poor, 5=Excellent)
1. Hypothesis identified
2. Sufficient background presented to understand t