Problem set #2
Due in class Thursday November 10th
Reconstitution of vesicle transport in cell-free systems was historically
carried out in the presence of isolated donor and acceptor Golgi stacks.
The donor stacks are
1. What is cell signaling? Types we spoke about in class? Why is it necessary?
When does it begin?
- Cell signaling is communication within a cell that governs basic cellular
activities and coordinates cell actions. In class, we spoke about paracrine,
the key to every biological problem must nally be
sought in the cell; for every living organism is or at
some 9me has been, a cell.
The three major divisions of the living world
Phagocytosis: a hu
Problem Set #1
PLX could be either inside or outside the cell; these data do not allow you to
distinguish between these two possibilities. PLZ is outside the cell, because PLZ
is rapidly transferred to the outside o
The hereditary informa/on determines the nature of the
whole mul/cellular organism
Dierent cellular func/ons and ac/vi/es
are based on the presence of dierent
genes within an organism
True or False. Why?
The Central Do
Problem Set 1
1.) Mouse. They are cheap and small; able to house plenty of them and not take up a
lot of room. They breed quickly. They have similar effects of corresponding
mutation found in humans.
2.) A. Immediately a
What is the most pressing issue today in biomedicine?
Clicker Ques9on #2
Phospholipids with short or unsaturated fatty acyl
a. decrease membrane fluidity.
b. increase membrane fluidity.
c. cause biomembranes to become thicker.
What do you hope to gain from this course?
Clicker Ques9on #1
The cell membrane is made up of a
A. Phospholipid bilayer
B. Semipermeable protein coat
C. Phospholipid unilayer
D. Osmo=c barrier
What is a concept that is confusing to you
in the course, so far?
Clicker QuesBon #5
O2 is bigger than Mg2+ and thus will diuse more slowly across a
False. A charged ion wil
The 3 classes of ATP-driven pumps
The pumping cycle of the Sarcoplasmic re;culum Ca2+ pump
What are some advantages and
challenges of cellular channels?
Aquaporins are permeable to water but impermeable to Ions by
What are some strategies that you will use to study
for exams in this course?
Clicker Ques?on #5
The major ATP-powered pump responsible for maintaining ion
gradients across the plasma membrane of mammalian cells is
Structure of the Nuclear Pore Complex (NPCs)
Electron Microscopic images of the NPC
Nuclear Pore Complex (NPCs)
Can transport up to 500 macromolecules per second
Can transport in both direc?ons
Size of 5000 Da or le
Clicker Ques3on #7
Consider a transcrip3on regulatory protein that has both a nuclear localiza3on and a
nuclear export signal and is normally found both in the nucleus and in the cytosol
Problem Set 3
A. Yes, all ion channels are involved in signal transduction. Ion channels are gated pathways in
which they open and close in response to stimuli. Depending on the signal acting upon the
Problem Set 4
Question 1: True/False
A. False. We do not become new people every single day. Our cells may divide into new cells
and old cells will be recycled, but not every single cell does this. For example,