BIS 104 PQ #7 COP Ia is a protein that is found in the cytoplasm of most eukaryotic cells. Investigators desiring to know more about the function of this protein decided to isolate and purify the protein from HeLa cells. HeLas are an immortal line of huma
BIS 104 assign week 5 spr 2010
Mukouyama, et al., Figure 5, pp. 697-699.
Nerves direct the patterning of vascular remodeling and arteriogenesis
Schwann cells are required for arterial differentiation and blood vessel- nerve alignment.
BIS 104 assign week 4 Spring 2010
Mukouyama: Figure 4 (results obtained from experiments using the Ngn mutant strain of mice) Practice questions: #13, 14 & 15. Reading in Karp: Chapter 4: Use of fluorescence recovery after photobleaching (FRAP) and single
In addition to studying the function of a specific gene by introducing it into cultured cells by transfection techniques, it is also possible to insert specific genes into live animals. These animals are known as transgenic animal models. (Karp, p. 770) A
One Way to Make a Polyclonal Antibody
Purify the antigen of interest. E.g., human erbB2 (a 185 kDa transmembrane protein) Inject antigen into host animal, e.g., rabbit or mouse that will perceive erbB2 as non-self i.e., a foreign protein. Periodically col
Please fill out this enrollment survey. It will provide me with an idea of the cell biology related topics that would interest you most. Circle all of the BIS and MCB courses that you have previously taken
BIS 103 PQ 9 Spring 2010 9. In a true scientific investigation, experiments designed to test a hypothesis must be accompanied by appropriate controls. a. Why are control experiments necessary for the proper intrepretation of experimental results?
BIS PQ 12 Spring 2010 12. Mukouyama chose the technique of scanning confocal fluorescence microscopy to visualize the immunostained skin samples in his study. What advantages does this particular technique have when compared to that of conventional fluore
BIS 104 PQ #17
a) What does the Semaphorin3A (Sema 3A) gene code for in normal (wild type) mice?
b) What do the PSNs in the limb skin of Sema3A mutant mice look like? That is, how are they different from PSNs in wild type mice?
c) How is the immunosta
BIS 104 PQ #16
In 2 3 concise sentences, describe the basic concepts stated in the Fluid Mosaic Model that attgempt to explain the basis for bio-membrane stgructure and ffunction relationships.
b) Summarize the observational and experimental eviden
BIS 104 PQ 15 Spring 2010 15. The lipid composition of membranes has a strong influence on the physical and physiological properties of membranes, such as the plasma membrane of the cell. Among the membrane lipids, phospholipids play a major role in deter
BIS 104 PQ 14 Spring 2010 14. Discuss the various factors that have an influence on the fluidity / viscosity of biomembranes. In your discussion, include the following factors: Lipid composition Lipid structure Cholesterol Temperature Lipid rafts
BIS 104 PQ 13 Spring 2010 13. To further investigate the possible role of PSNs in mouse limb skin arteriogenesis, Mukouyama made use of the Neurogenin 1 / Neurogenin 2 (Ngn 1-/- : Ngn 2-/-) double homozygous mutant strain of mice. It has been established
BIS 104 PQ 11 Spring 2010
11. Define the following concepts or terms that are important aspects of microscopy:
a) magnification: What is the formula for calculating the effective magnification of a microscope?
b) resolution: What is the formula for calcul
BIS 104 PQ 10 Spring 2010
10. In their second series of experiments, Mukouyama, et al. immunostained mouse embryo limb skin samples from 3 different developmental sta Ges (E13.5, E14.5, and E15.5). They immunostained these tissues for arterial markers (ep
BIS 104 PQ 8 Spring 2010 8. It has been hypothesized that some human breast cancers arise when breast epithelial cells begin to express abnormally high levels of a growth factor receptor, erbB2, on their plasma membranes. The erbB2 molecule is a transmemb
Infectious Disease Genomics
November 18, 2016
Human disease module 1:
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Host genetic/genomic diversity
affects infection outcome
(e.g. life or death)!