KEY EXP 0 - CDE: Calculations and Points for Discussion (entered values are samples)
1. Attach the microplate print-out.
(i) Calculate the dilution series for each of the bromophenol blue dilutions created in the microplate.
Columns 1 4 was a 1/2-dilution

Exp 1 Key
Part A. Buffer Preparation
1. pH = pKa + log ( A x Vol A x [A] / HA x Vol HA x [HA]) and
0.10 M = [A] = [HA]
7.4 = 7.22 + log (0.445 x Vol A / 0.744 x 19 ml); 1.51 = 0.445 x Vol A / 0.744 x 19 ml
Vol A = 48.0 ml ~ 48.1 ml (not what is required f

Experiment 5 Write-up Key
MCB 120L, Spring 2014
DNA Quantification example values
1. Nanodrop printout attached
2 (a). Concentration of the column purified PCR product:
An absorbance value of 1.0 corresponds to 50 g/ml of double stranded DNA at 260 nm in

KEY EXP 0: CALCULATIONS AND POINTS FOR DISCUSSIONS (entered values are samples)
For the end of Day 1 (Parts A & B)
1. Report the best results from Table 0-1 in the table below:
Values consistently below 0.3 are
usually a result of the cuvettes
being place

Lecture 9 MCB 120L S2014
Announcements:
Quiz 1 Answer Key: Posted in Briggs Hall Glass Case (Rm 139 Briggs)
Regrades: Before Friday Apr 25th 9 am. Must use regrade form
(available in 145 Briggs Hall).
Lab Practical #1: Today & Tomorrow Apr 23-24th. Open B

Lecture 14 MCB 120L S2014
Announcements:
Quiz 2 Returned in lab (AVE 7.1; Hi 9.5; Lo 2.5)
Lab Practical #1: Returned in lab this week.
Answer Keys: Posted in Briggs Hall Glass Case (Rm 139 Briggs)
Quiz 3 Friday May 9th (Exp 3-focussed)
Todays Lecture:
Exp

Lecture 12 MCB 120L S2014
Announcements:
Friday: Quiz 2 (through Exp 2)
Section A02 (in the lab)
Section A01 (in Lecture Hall)
In Lab this Week: Expts 4 (cont) & 5 (PCR)
Todays Lecture:
Purification Calculations
A real life protein purification from algae

Lecture 13 MCB 120L S2014
Announcements:
Quiz 2 Returned in lab (AVE 7.1; Hi 9.5; Lo 2.5)
Lab Practical #1: Returned in lab this week.
Answer Keys: Posted in Briggs Hall Glass Case (Rm 139 Briggs)
Quiz 3 Friday May 9th (Exp 3-focussed)
Todays Lecture:
Exp

Lecture 8 MCB 120L S2014
Announcements:
Quiz 1 Returned in Lab Section (AVE 5.4; HI 10; LO 1)
Answer Key: Posted in Briggs Hall Glass Case (Opposite Rm 139 Briggs)
Regrades: Before Friday Apr 25th 9 am. Must use regrade form
(available in 156 Briggs Hall)

Lecture 10 MCB 120L S2014
Announcements:
Experiment 4 Next Week (New Lab Partners)
Experiment 3C Data Update Next Week (Delay due date by 1 lab)
Complete Exp 3AB & D Analysis by Monday and Tuesday
Todays Lecture:
Lactate Dehydrogenase (LDH) Assay and Puri

Experiment 4 write-up Key
Table 4.5 LDH Activity as a Function of the % Ammonium Sulfate Concentration: Supernatants
Total vol
Sample
LDH activity Total protein
Specific
% soluble*
Tube
after AS
300 l
Total IU
mg
activity
(ie % yield)
added, l
Bovine Musc

Exp 3 Key
MCB 120L, S14
Part A. Enzyme activity as a function of pH ([enzyme] and [substrate] are constant)
1. Typical graph:
Rate of AP vs pH
0.025
0.02
IU
0.015
0.01
0.005
0
6.5
7
7.5
8
8.5
9
9.5
10
pH
IUA = (A410 corrected/ min)(106 mol/mol)(0.001 L) /

Welcome to MCB 120L S2014
Introductions: Course Instructors (3015/6 SLB)
Dr. Clark Lagarias, Instructor in charge, MW pm (A02)
TAs Anh Le, Tamsen Polley
Drs. Larry Morand & Dinesh Kumar, TuTh pm (A01)
TAs Matt Tan, Jillian Ng, Neeraj Lai
Enrollment
Show u

Lecture 6 MCB 120L S2014
Announcements:
Quiz 1 Friday Apr 18th OPEN BOOK Expts 0 and 1 (lectures 1-4)
TuTh pm A01 (Morand) in Surge III Room1309
MW pm A02 (Lagarias) in 3015 and 3016 SLB
Bring calculators, pencils/pens, erasers; no internet/cell phones.
T

Lecture 11 MCB 120L S2014
Announcements:
Friday: Quiz 2 (through Exp 2)
Section A02 (in the lab)
Section A01 (in Lecture Hall)
In Lab this Week: Expts 4 & 5
Todays Lecture:
Ammonium Sulfate Fractionation
Purification Calculations
How do we purify an enzym

KEY
Exam 3, Friday 07 March
Abs = lc
20 points
MCB 120L, Winter 2014
phycocyanobilin (PCB): (652 nm) = 132,000 M-1 cm-1
Shimadzu 1 cm path length
1. The GAF-4 domain is to be cloned and expressed from an expression plasmid, and then purified on a nickelaf