Experiment 1: LabView for Analytical Instrument Control and Data Analysis
Introduction: LabView is a graphical programming language that can be used to control
scientific instruments and analyze the data produced by those instruments. In this experiment,
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Electronics: Why do we care?
Two main reasons
1. Every instrumental measurement involves a transduction
step that converts a chemical/physical response into an
electrical signal.
Need to ensure that signal is a good reflection of the
response
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Atomic system and spectroscopy
Chapter 8
Introduction
What is atomic spectroscopy?
To determine elemental composition by its
electro magnetic or mass spectrum.
1
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Introduction
Sample is atomized (atoms/ions)
- atomization
absorption,
Laboratory notebook and Laboratory Report
Laboratory Notebook
All data should be entered directly in a bound notebook. Do not use loose-leaf binders or
water-soluble ink. To make corrections, draw a line through the text, DO NOT ERASE.
For each experiment
Determination of unknown concentration using standard addition method
Unknown concentration can be determined using the standard addition method by plotting the
absorbance against concentration of the added standard in the analyzed samples. Below is an ex
Multivariate Statistics
More than one variable with uncertainty/error
y = mx + b; fit m and b simultaneously
for n fitting parameters, need minimum of n data points to solve
if have N > n, then also can estimate uncertainty in parameters
m = q C.L.1
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Molecular Spectroscopy
Chapter 13 & 14
Beers Law for Mixtures
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Chemical effect
analyte associates, dissociates
or reacts to give molecule
with different
e.g. pH Indicator
Hin (color 1) H+ + In- (color2)
570 nm 430 nm
color2
Example
Determination of unknown concentration using standard addition method
Unknown concentration can be determined using the standard addition method by plotting the
absorbance against concentration of the added standard in the analyzed samples. Below is an ex
Name
Student ID
EY
Chemistry 105
Midterm Exam
May 5th, 2016
Dr. Tomoyuki Hayashi
Instructions:
1. You will have 50 minutes to complete the exam. You may use a calculator. No collaboration is
allowed.
2. The last page contains a periodic table and some use
Liquid Chromatography
Liquid Chromatography
Chapter 28
Column Efficiency Improves with
Smaller Packing Particles
Plate height H changes with flow rate (Fig 268)
A dp
CM d p 2
Column Efficiency Improves with
Smaller Packing Particles
Liquid Chromatograph
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Data Analysis
1. Basic data analysis
2. Examples more about errors
3. Normal error curve
4. Confidence Limit
5. Linear least square fit
1. Basic data analysis
a. Precision and Accuracy
b.Absolute error
c. Random error
d.Systematic error
1
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Semiconductor Lasers
Chapter 7
Emission
An atom drops from an initial level i to a
lower-energy final level f by emitting a photon
energy of emitted photon
hf =
hc
= Ei E f
h, Plancks constant; c, speed of light; f, frequency; , wavelength
1
4/
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pH meter
Now
Eb = E1 E2 =
a
RT a1
ln = 0.0592 log 1
F
a2
a2
a1=analyte
a2=inside ref electrode2
If a2 is constant then
Eb=L+0.0592loga1
=L-0.0592pH
pH = - log [H+]
Ecell = Eref1 + Eb + Eref2
Since Eref1 and Eref2 are constant
Ecell=constant-0.0
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Che105
Chromatographic Separation
Chromatographic Separation
Chapter 26
1
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Introduction to Chromatographic
Separations
Chromatography
Sample transported by mobile phase
Electrostatic or van der Waals interactions
Some components in sampl
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Population mean and Sample mean
Population Mean ()
N
x
xi represents the value of the ith measurement.
i
= lim
i =1
N
N
For a very large (theoretically infinite) number of
measurements, one can determine the true mean, ,
exactly, and the spre
Probability and Statistics
Probability is the likelihood or chance that
something is the case or will happen.
Theoretical probability is used extensively in areas
such as statistics, mathematics, science, and
philosophy to draw conclusions about the lik