Robert H. Rice
Department of Environmental Toxicology
Study of Epidermal Keratinocytes in Culture
Dependence of Growth on EGF
You need to work what you are working with - Authentication
Lineage or Tissue markers
Cell surface antigens
Widely used to sort out hematop
Genetic modifications of
Recombinant protein production
Study gene/protein function and regulation
Modify cell properties (immortalization)
Viable freezing of biological material and
subsequent storage at ultra low temperatures.
Cryobiology: study of the effects of low
temperature on biological systems, such as
cells or organisms. Fr
Cell lines vs primary cultures
Primary culture: A culture started from cells, tissues, or organs taken
directly from organisms.
Cell line: A cell line arises from a primary culture at the time of the
first successful subcul
Media, and Substrates
*Slides from PJ Ross, modified by MJ Mienaltowski
Michael J. Mienaltowski, DVM, Ph.D.
Freshney, R. I., Culture of Animal Cells, 6th edition, (2010) Chapters 7-10.
When considering cell culture