Protein Function:Protein Binding Introduction
Ch2, Page 36 Ch3, Page 73-79, 82-86
Proteins are very versatile.they can perform a variety of
"jobs" in the cell
How do proteins accomplish their function?
Ghost in Your Genes Worksheet
1. Genes are important in determining what you look like. What else plays an
2. What is the orthodox biological view of inheritance? Aka, how do your parents
inuence how you look?
Inheritance of se
LS 3 Notes
Week 1 Lec 1
DNARNA Protein (central dogma of molecular biology)
Protein is the gene product (gene product is on the protein)
DNA is the blueprint and RNA is the messenger
DNARNA(transcription, can go reverse RNADNA
Central Dogma of Molecular Biology
DNA and RNA viruses
Modification of Central Dogma
Central Dogma* of Molecular Biology
-how do we study proteins?
How to separate different cellular components?
and different proteins?
(centrifugation, chromatography, SDS-PAGE, etc)
II. Detection (immunological techniques)
How to detect a single protein from a
1. Nucleosides, nucleotides, and polynucleotide
2. DNA double helix
Anti-parallel nature of the double strand
Complementary nature of the double strand
3. Other structural properties of DNA
DNA Denature and renature
RNA Structures and Functions
message RNAused as template for protein
translation, usually a linear structure
transfer RNAused to bring amino acids for
translation reaction. tRNA has an unique cloverleaf like structure,
1. Semi-conservative replication
how to test Watson-Crick hypothesis?
Meselson-Stahl experiment (1958)
2. Replication process
Replication origin and supercoil problem
Replication fork and bi-directional replication
Leading strand vs laggin
1. "Central Dogma"
Central dogma, originally proposed by Francis Crick,
states that the flow of the gene
Function of proteins
-most work in the cell are done by proteins,
how do proteins work?
1. Protein family
Why different proteins have different
2. How do proteins work?
3. How are proteins regulated in the cell
1. Protein f
Discussion Section 3
Name _ UID _
Name _ UID_
1. Beta-pleated sheets are found in many proteins.
a) How is the hydrogen bonding between two strands of a beta-pleated sheet
different from the hydrogen bonding holding two strands of DNA together?
Discussion Week #4
Transcription consists of 3 stages:
Emzyme: RNA Polymerases (I, II, & III), e.g. RNA Pol III
RNA Polymerases bind to 10, 35 and 50 promoters (rela
Regulation of Chromatin Structure
ATP dependent enzymes that move or loosen
Post-translational modification of histones
Activating modifications (such as acetylation)
Repressive modifications (methylation at specific
DNA polymerase exonuclease activity is
required to remove RNA primers. Which
exo performs this functions?
5 to 3
3 to 5
Which of the following
enzymes break the
phosphodiester backbone and
Ligase then remake it?
Genome structure and mutation
Gene and genome structure
Different types of mutations
Effects of mutations to gene expression and functions
What cause cancer?
O rg a nia tio na nd c o nte nto fth e h um a ng e no m e
Life Science 23L
July 26, 2016
Effect of Caffeine on Oxygen consumption Rate of Goldfish
Living organisms are capable of moving, growing, and expending energy because of the
What is the carrier of genetic
information? DNA or Proteins?
Griffith Experiment (1920s)
Avery, MacCleod, McCarty Experiment (1940s)
Hershey Chase Experiment (1950s Bacteriophage
T2 and Radioisotopes)
Griffith Experiment (1920s)
use combination of about 13 STRs to prof
tata box rich in AT pairing
-bound by TBP
dont have to worry about factors
TFIIH - has helicase which helps unwind helix by using ATP
LS3 Midterm II Part 2
Eukaryotic RNA polymerase is larger
and contains additional polypeptides
Pol II is the main gene encoding pol.
Pol I and Pol III transcribes RNAs that
do not get translated!
Eukaryotic core promoter refers to
LS3 Midterm II Review
1. Sigma binds to DNA by recognizing
specific sequences called promoters.
In the closed complex sigma
recognizes -35 and -10 through
H bonding between alpha helix and
major groove (helix turn helix mo