Microbiology in the post-genomic era
Duccio Medini*, Davide Serruto*, Julian Parkhill, David A.Relman, Claudio
Donati*, Richard Moxon|, Stanley Falkow and Rino Rappuoli*
Abstract | Genomics has revolutionized every aspect of microbiology. Now, 13
Functional Characterization of the Antibiotic Resistance Reservoir in
the Human Microflora
Morten O. A. Sommer, et al.
Science 325, 1128 (2009);
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Propionibacterium acnes Strain Populations in the
Human Skin Microbiome Associated with Acne
Sorel Fitz-Gibbon1,8, Shuta Tomida1,8, Bor-Han Chiu1, Lin Nguyen1, Christine Du1,2, Minghsun Liu3,
David Elashoff2, Marie C. Erfe4, Anya Loncaric
Genome analysis of multiple pathogenic isolates
of Streptococcus agalactiae: Implications
for the microbial pan-genome
Herve Tettelina,b, Vega Masignanib,c, Michael J. Cieslewiczb,d,e, Claudio Donatic, Duccio Medinic, Naomi L. Warda,f,
Samuel V. Angiuolia
ARE 112 Fall 2017
University of California - Davis
Department of Agricultural and Resource Economics
Managerial Economics Program
ARE 112 Organization Management
Monday and Wednesday 4:10 PM to 5:50 PM 1100 Social Science
The Authors Journal compilation 2011 Biochemical Society
Essays Biochem. (2011) 51, 177191; doi:10.1042/BSE0510177
Mechanisms of adaptation in
the intestinal parasite Giardia
Hugo D. Lujan1
Laboratory of Biochemistry and Molecular Biology, Sch
Historical Profiles and Perspectives
Giardia and Vilem Dusan Lambl
Laboratory of Molecular and Cellular Immunology, Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Prague, Czech Republic
Giardiasis is a neglect
MIMG 168 - Molecular Parasitology
Prof. Patricia Johnson
Prof. David Campbell
Dr. Michelle Castelletto
Prof. Elissa Hallem
Prof. Peter Bradley
Introduction to Microbiology
MIMG 101 Syllabus
Umesh Ahuja; [email protected]
Office: BSRB 210C
Office hours: Tue, 2-3; Wed, 2-3
The best way to contact me is by email.
Kent Hill; [email protected]
Office: 4801A M
Basics of Light Microscopy
Staining & Gram Stain
How does it differ from brightfield?
What can we view with this?
Darkfield Microscopy how does the angle of the light differ?
Prokaryotic Membranes & Cell Walls
Explain how different membrane structures affect the
function of membranes.
Bacterial versus Archeal Membranes
Predict how prokaryotic membranes will change in
response to environmental conditions.
Effect of temperat
Cultivating and Identifying
1. Choice of environmental sample
2. Analyzing the sample microscopically - Gram Stain
Liquid vs Solid
4. Isolating a pure culture streak purification
5. Identifying the bacter
MIMG132 POST TRANSCRIPTIONAL CONTROL, NUCLEAR TRANSPORT, AND EUKARYOTIC CELL CYCLE
(10) 1. Alternative cleavage and polyadenylation can be coupled to a change in splicing to
allow the use of two alternative 3 terminal exons and the creation of two possibl
MIMG132 GENES, CHROMSOMES, AND TRANSCRIPTIONAL CONTROL OF GENE EXPRESSION
1. Bacterial mRNAs often encode multiple proteins. Describe why this generally does not occur in
animal and plant cells. What step of the gene expression pathway is fundamentally di
Questions for Discussion Week 10:
1. The gypsy moth is a pest of hardwood tress in the eastern United States. You want to
know whether this gypsy moth has a bacterial endosymbiont. You are able to PCR amplify
the gene for 16S rRNA of the bacterium, En
Question from Discussion Week 3
1. Magnetosomes, cytoplasmic membrane-encased vesicles of iron oxide,
were revealed through cyroelectron microscopy to initiate through
invagination of the inner membrane (see picture).
Questions for Discussion Week 8:
1. Expression of a microcin antibiotic by Streptococcus pneumoniae is off at low density
cells and on at high density cells. This is due to a small peptide, PhrA, that S. pneumonie
secretes into the extracellular mediu
Question from Discussion Week 5
1. You are an undergraduate researcher in a lab that studies linear dsDNA genomes of
Herpes viruses, which replicate by rolling circle replication. A new student has been given
Questions for Discussion Week 7:
1.You have identified an RNA sequence that can bind cyclic di-GMP, a small molecule found
in bacteria. This RNA sequence is found in many bacteria species, including Clostridium
difficile where it is predicted to form a lo
Questions for Discussion Week 6
1. You have isolated a virus that infects eukaryotic cells and has a single ssRNA(-) genome.
You find that one of the viral proteins is similar at the amino acid sequence level to other
characterized proteins with endonucle
chromatin is added to
an egg extract, a
forms around the
sperm chromatin and
decondense (30 min),
These results indicate that cyclin B is the only protein that must be
synthesized in the egg extract in order for cycling mitotic events to
The APC/C (anaphase promoting complex)/cyclosome is the E3 ubiquitin protein
ligase that polyubiqu
Meiosis: Generation of haploid
germ cells (egg and sperm).
Figure 19.38 Meiosis.
Figure 19.37 Comparison of the main features of mitosis and meiosis.
During meiosis, expression of G1 cyclins is repressed, blocking the
normal progression of G1 in cells as
Figure 19.1 The fate of a single parental chromosome throughout the eukaryotic cell cycle.
Phases of the eukaryotic cell cycle
Figure 18.36 The stages of mitosis.
Centrosomes duplicate by G2