BE 100. BIOENGINEERING FUNDAMENTALS
SPRING 2017
Additional Problems before Exam 1
Not to be Turned In
1. You are working in a drug delivery company, and your job involves loading a particular
anticancer drug into vesicles such as the one shown in the figu
BE 100. BIOENGINEERING FUNDAMENTALS
SPRING 2017
Additional Problems before Exam 2
Not to be Turned In
1. You are working in a biotechnology company trying to deliver a small molecule drug to
cancer cells. When you first started at the company, you investi
BE 110:
Bioreactors (cont.)
Lecture #8
Biotransport and Bioreaction Processes
Prof. Daniel T. Kamei
3
Kinetics of receptor/ligand binding
Bioreactors (cont.)
Outline
Prof. Daniel T. Kamei
Rearrange
Recall Eq. (1)
Q
1
CSF CS
X
V
YX / S
Balance on
cells
X
BE 110:
Receptor/Ligand Binding (cont.)
Lecture #9
Biotransport and Bioreaction Processes
Prof. Daniel T. Kamei
3
Kinetics of receptor/ligand trafficking
Kinetics of receptor/ligand binding (cont.)
Outline
Prof. Daniel T. Kamei
kf
Plasma membrane
L+R
C
kr
BE 110:
Biotransport and Bioreaction Processes
Lecture #3
Example: Drug Delivery (continued)
Example 6.8 in Truskey et al.
A porous sphere of radius R contains a drug to treat a
particular disease. The sphere is a porous polymer to allow
controlled releas
BE 110:
Lecture #2
Biotransport and Bioreaction Processes
(x0-l,y0,z0)
(x0,y0+l,z0)
x, y, and z directions.
Molecules move at speed u in each of the
Lattice spacing is l (distance of one jump).
any one of the six adjacent sites.
Collection of molecules at
BE 110:
Lecture #4
Biotransport and Bioreaction Processes
Need to incorporate convection and/or reduce the length scale.
See that a protein or virus diffusing only 1 cm can take an
extremely long time.
Always have an idea of diffusivities and length scale
BE 110:
Biotransport and Bioreaction Processes
Lecture #5
Combining Reaction Kinetics and Diffusion
Prof. Daniel T. Kamei
3
Diffusion and reaction in porous media
Damkhler number in diffusion and reaction problems
Combining reaction kinetics and diffusion
BE 110:
Biotransport and Bioreaction Processes
Lecture #6
Diffusion and reaction in porous media (cont.)
Prof. Daniel T. Kamei
3
Material related to Exam #1
Point regarding ODEs that can be solved analytically.
Diffusion and reaction in porous media (cont
BE 110:
Biotransport and Bioreaction Processes
Lecture #10
Receptor/Ligand Trafficking (cont.)
Prof. Daniel T. Kamei
3
Kinetics of receptor/ligand trafficking (cont.)
Outline
A few signaling concepts
Combining diffusion with receptor/ligand binding and
tr
BE 110:
Reaction Kinetics
Bioreactors
Will now move to
Tanks
Convection
3
Mass Action Kinetics
Biotransport and Bioreaction Processes
Lecture #7
Current State of the Course
Diffusion
1D Steady-State
Prof. Daniel T. Kamei
Short review of the integrating fa
Name:
Number:
BE 100: BIOENGINEERING FUNDAMENTALS
WINTER 2017
Exam 2
February 28, 2017
1. Consider a gas that can be described by the following Redlich-Kwong equation of state
/
where P is the pressure, R is the ideal gas constant, T is the absolute tem
BE 100. BIOENGINEERING FUNDAMENTALS
WINTER 2017
PROBLEM SET 2
Due January 26, 2017 (Thursday)
1. According to BLAST, what are the names of the ten proteins that are most homologous to
bovine serum albumin?
2. Short answers
a. Why are hydrogen atoms missin
BE 100. BIOENGINEERING FUNDAMENTALS
WINTER 2017
PROBLEM SET 6
Due February 23, 2017 (Thursday)
1. You have an aqueous solution containing a protein of interest, sodium chloride (which
dissociates completely), and glucose. You want to remove glucose from t
BE 100. BIOENGINEERING FUNDAMENTALS
WINTER 2017
PROBLEM SET 8
Due March 9, 2017
1. Consider an aqueous solution containing two types of polypeptides (A and B). The
following self-assembly reaction occurs in this solution.
n A type A polypeptide molecules
BE 100. BIOENGINEERING FUNDAMENTALS
WINTER 2017
PROBLEM SET 5
Due February 16, 2017 (Thursday)
1. Short answers
a. At constant U, V, and n, which state function do you minimize or maximize?
b. What is a colligative property? Name two colligative propertie
BE 100. BIOENGINEERING FUNDAMENTALS
WINTER 2017
Additional Problems before Exam 2
Not to be Turned In
1. One particular charged polymer (polymer i) is added to the left-hand side compartment of
a diffusion cell that is filled with permeable water. The mol
BE 100. BIOENGINEERING FUNDAMENTALS
WINTER 2017
PROBLEM SET 1
Due January 19, 2017 (Thursday)
1. Estimate the pI of the peptide Asp-His-Glu-Lys-Arg-Thr. Asp is considered the first
residue, since it has the amine group at the end, while Thr is considered
BE 100. BIOENGINEERING FUNDAMENTALS
WINTER 2017
PROBLEM SET 7
Due March 2, 2017
1. You are analyzing a nonionic surfactant that has a hydrophilic head that strongly
hydrogen bonds with water. Do you expect gmic to increase or decrease with an increase
in
BE 100. BIOENGINEERING FUNDAMENTALS
WINTER 2017
Additional Office Hour Problems for Week 8
1. You are working in the drug delivery division in a pharmaceutical company. You are
studying the delivery of a hydrophobic small molecule that you are planning on
BE 100. BIOENGINEERING FUNDAMENTALS
WINTER 2017
Additional Office Hour Problems for Week 7
1. A single-phase solution containing polyethylene glycol (a polymer) and water can phase
separate into two liquid phases ( and phases) with an increase in temperat
BE 100. BIOENGINEERING FUNDAMENTALS
WINTER 2017
Additional Office Hour Problems for Week 9
1. Is the surface tension of water greater than, less than, or equal to the interfacial tension
between oil and water? Please explain.
2. Given the following virial
BE 100. BIOENGINEERING FUNDAMENTALS
WINTER 2017
Additional Office Hour Problems for Week 6
1. Derive an expression for the partial molar volume of component 1 given the following
expression for the molar volume of the binary liquid solution:
V a 0 a1X 1 a
BE 100. BIOENGINEERING FUNDAMENTALS
Winter 2017
Additional Office Hour Problems for Week 10
1. Consider the model developed by Linus Pauling for hemoglobin binding to
oxygen. The energy level representation for this model is shown below. In this
model, th
NAME:
Introduction to Tissue Engineering (140 pts total)
Final Exam Spring 2015
This exam must be turned in no later than 3:00 pm on Friday, March 20, 2015, to
Professor Kaskos Office in room 4121K Engineering V. If you do not turn your exam in
on time, y
Jeopardy Answers
General
1) Name 2 tissues that can regenerate spontaneously, and two that cannot.
Regenerative Tissues
Epithelial
Connective
Poorly- or Non-Regenerative Tissues
Nervous
Muscle
2) Define the terms autograft, allograft and xenograft, an
Name:_
Introduction to Tissue Engineering WINTER 2016 MIDTERM EXAM: 100 pts
Definitions (3 points each, choose 4, 12 pts total)
RNA interference: silencing of gene expression triggered by the presence of doublestranded RNA homologous to portions of the ge
Jeopardy Questions
General
1) Name 2 tissues that can regenerate spontaneously, and two that cannot.
2) Define the terms autograft, allograft and xenograft, an describe a limitation of
using each
3) Name the fou
1. Scaffolds
a. Name three properties of a successful scaffold
b. 3 Classes of materials used as scaffolds
c. Explain which class of material is used most often for scaffolds and
why?
d. Name 2 ceramics used as scaffolds
e. Name 2 metals used as scaffolds