Biol. C2005/F2401 Handout 2E
A minimal medium for growth of E. coli (sources for the most needed atoms C, H, O, N,
K, Mg, S
glucose, a sugar
7-A: Derivation of the Michaeli-Menten equation describing the kinetics of enzymecatalyzed reactions.
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Derivation of the Michaeli
Methods based on size for separation of large molecules
When you put a particle in a centrifugal field, it is acted upon by the centrifugal force, which is proportional to the
molecular weight (M), to the square of the speed
The Polarity of Molecules
We have seen that atoms differ in their capacity to "hold onto" their electrons; some gain electrons, some lose
electrons. Certain atoms, oxygen and nitrogen, for example, do not have sufficient electron-attractive power to
Review Questions for Exam #3 = Exam #3 of 13
For all the questions on this exam, circle all correct answers for each question. There may be one or more than
one correct answer. Then explain all your answers!
1. You have mRNA from eukaryoti
Last five digits of SS# or ID# _
Class _ (C2005) (F2401)
Seat No. (if available) _
Intro Bio Exam #1 September 29, 2005
There are 4 pages with questions.
Pages will be separated for grading. To receive proper credit for your answer, you must
Last five digits of SS# or ID# ANSWER KEY_
Class _ (C2005) (F2401)
1) Consider the diagrams of the chair form of beta-glucose and the Haworth projection of beta-glucose and
beta galactose. e.
a) In alpha-galactose, the anomeric carbon is
f(A) = frequency of allele A, f(AA) = frequency of genotype AA, etc.
Call f(A) = p; f(a) = q. Then p + q = 1 since every allele is either A or a. At equilibrium:
f(AA) = p ; f(aa) = q , and f(Aa) = 2pq, and p + 2pq + q = 1,
- How to measure an RF with diploids
Suppose you want to measure the RF between gene Alpha (alleles A & a) and gene Beta (alleles B and b).
The obvious thing to do is to cross AABB X aabb (parents) AaBb F1 (or cross AAbb X aaBB), and then to
Intro Bio Columbia Univ.
Important organic chemical FUNCTIONAL GROUPS found in biological molecules (R = any organic group):
sugars :e.g., glucose
PECDM B/NIVT 5/1/24
W599" (343' m'l'erST
_ WEIGHT DNA
CLEAVE wnu RtsmcnoN ENIYMES (3 dis? 0?5>"' ' 9i,- 3 d l? DAM
m k. 00
V J c: l antral lJU/
\ it / , Same; 42an m C
AGAROSE GEL Ettcnaomont% -=-
._._I in "
A CLONING VEHiCLE-
RESISTANCE T0 BOTH
Amp AND Tot
PLASMID CUT PLASMJD CUT
PLASMID CONFERS PLASMID CONFERS
RESISTANCE ONLY T0 In RESISTANCE ONLY TO Amp