Dr. Helen WiersmaKoch
Email: [email protected]
EMAIL IS THE PREFERRED METHOD OF CONTACT
Office Location: N-111
Office Hours: Office Hours will be posted on the door and
Western Blot Using Myosin Light Chain Protein From Fish Muscle
Myosin is a hexameric protein found in muscle tissue. It consists of heavy and light
chains that are the primary determinants of contractile performa
Biotech II Lab
Powerful tool in research and clinical diagnostics
A surefire way to identify the presence of
Two Basic Steps:
1. SDS-PAGE: separate proteins based on mass
2. Blot: transfer proteins from gel to
Tracking a Disease Outbreak
Biotech II Lab
ELISA stands for enzyme-linked immunosorbent
A powerful antibody-based test
Diagnose diseases such as HIV/AIDS
Track pathogenic agents in water, food, and the air
Donald Voet Judith G. Voet
Charlotte W. Pratt
Ionic and polar substances can only traverse the membrane
through the action of specific transport proteins
Exam 2 (Ch. 4 7)
Please turn in the completed study guide on test day.
1. Define zwitterion.
A neutral molecule with a positive and negative electrical charge
Multiple negative and positive charges can be present
Genetic Engineering of Plants
Recombinant DNA technology is also an
important tool for the direct genetic
manipulation of plants.
There are a number of effective DNA delivery
systems and expression vectors that work with
a range of plant cells.
Engineering Plants to
overcome Biotic and
The cost of maintaining genetically
engineered insect-resistance crop is lower.
The biological insecticides are highly specific
and considered to be non-hazardous to
human and ot
Engineering Plant Quality
Modification of Plant Nutritional Content
Traditional breeding approached are both
difficult and slow.
It is often limited by the existing genetic
content of cross-breeding strains.
Genetic engineering techniques spe