Bio98 Winter 2016 Mid-term 1
Version A Student ID:
01. For each molecule shown, in the box below the molecule, write in the amino acid it depicts. If the
molecule shown is not a standard amino acid, write in NS.
Version It Cys, Lys, Giy, NS
Version B: Asn
Bio 98A Midterm Exam
Name _ (4) _
For the following two ligands, what type of forces could be involved in their non-covalent binding to a protein's active site? (do not include van der Waals forces) leucine ionic or charge-charge or elect
Bio 98A Midterm Exam 2/11/08
ID # _
TA Use Only
A term used to describe a molecule that contains both a hydrophobic and a hydrophilic region . a b c d e clathrate amphipathic micelle bipolar amphoteric
The predominate form of an important
Student ID: _
A person who takes a flight from Orange County (elevation ~ 100 feet) to Denver (elevation ~4500 feet) might
show increased glycolysis for a few hours (even if his nutritional status is the same as it would have been had this
Reading Guide: Week 2
What is an active site?
What stabilizes the transition state in active sites?
What contributes to the decrease of the transition state energy in
Active sites, Amino acids:
What does entropy redu
Reading Guide: Week 6
Differences between Hexokinases 1-4
How does Glucose-6-Phosphate affect Hexokinase isozymes?
What affects transcription of Hexokinase 4
Know circumstances where Glycolysis would be favored or
Student ID: _
Questions of taste and religion aside, and based on bioenergetics, is a vegetarian diet better for the environment?
Based on what you know about carbon fixation and consumption, does it make sense for meat to cost almost the
same as fruit
Reading Guide: Week 5
What do enzyme inhibitors do?
What is the difference between irreversible and reversible
What are mechanism-based inactivators? List an example.
Does the irreversible inhibito
Student ID: _
For some strange reason, after a really late Friday night party, you and your friend decide to mix a gallon of milk
with a gallon of orange juice (for orange-milk shots, maybe?). You end up with a gross-looking curdled bucket of
1. You are trying to purify the enzyme Rubisco. The SDS-PAGE gel of
the different purification steps are shown. Exactly 25g of TOTAL
protein was loaded into each lane. Based on this, answer the following
a) What is the reaction catalyzed by thi
Reading Guide: Week 3
What is essential before a protein's properties and activities can be
Applications of protein
What are some properties that
Student ID: _
I am trying to purify an enzyme (E1) that catalyzes the reaction A+B C+D. First, I
must have some way to tell whether E1 is present in my sample. What could be TWO
ways to do this ?
The enzyme Alcohol dehydrogenase
Bio. 98 Midterm Review
Instructors: Poulos/Cumsky Peer Tutor: Michael Chau
Format of this session
This review session:
Focuses on: A few important concepts per lecture Common questions associated with topics Does NOT focus on: Memorization-type
Bio 98A Midterm
If one combines 0.1 moles of NaH2PO4 and 0.2 moles of Na2HPO4 in 900 ml of water, the final pH will be _7.16 or 7.2_. -
log R = D, so log (0.2/0.1) = 0.301, The basic form prevails around the pKa of 6.86 So
Reading Guide: Week 8
What is the experimental evidence that ATP synthesis requires a
ATP Synthase and ATP
generation, ETC Part 2:
How does the affinity of ATP Synthase for ATP result in the
formation of ATP from AD
Reading Guide: Week 4
What is the purpose of mass spectrometry?
What are the molecules that are to be analyzed called?
What is the significance of the m/z ratio? How does it help
distinguish between ions?
Mass spec to study
proteins: Pages 98
Reading Guide: Week 9
How is fatty acid used differently in muscle and adipose tissue?
Under what conditions are triacylglycerols mobilized in adipose
tissue? How does this occur?
How do hormones epinephrine and glucagon regulate fatty acid
Reading Guide: Week 7
How do varying levels of CO2 affect the TCA process?
Can the CAC function in anaerobic conditions?
Citric Acid Cycle:
What other pathways can the CAC be linked to (what makes it so
central to metabolism)?
Reading Guide: Week 10
How does nitrogen fixation occur? Is this a universal process that
occurs in most organisms?
Amino acid metabolism
Pages 695-728, 881-891
Describe what occurs in the symbiotic relationship between
Bio98 Winter 2016 Discussion section #1
1. Consider the reaction CH3CH2CH3 + H2O CH3CH2CH2OH + H2. The uncatalyzed
reaction has the following characteristics:
Reaches equilibrium in 60 minutes
G0 = 12 kJ mol-1
Based on this, answer the following questions
Reading Guide: Week 1
Identify and describe the four types of weak bonds.
Weak bonds: Pages 54-55
What are some processes or structures that use weak bonds?
What characteristic makes carbon significant in the chemistry of