0.25 / 0.25 points
Which of the following describes the relationship between the strengths of hydrogen and covalent bonds?
Hydrogen bonds are always stronger than covalent
Hydrogen bonds and covalent bonds have equivalent
To perform kinetic analysis of adhP, pyrazole was used as an inhibitor in the
kinetic analysis. A competitive inhibitor competes with the substrate to bind to the
active site, therefore limiting the number of enzyme molecules accessible to the
Plasmid DNA that came from the TOPO cloning and transformation reaction
was purified before restriction digest. A resuspension buffer with RNase A is used to
provide an ideal pH for ensuing lysis and to remove RNA from the plasmid DNA to
The gene adhP was purified by running an affinity chromatography column.
In affinity chromatography, beads are modified with a substrate that binds to the
protein of interest. A column with Nickel-NTA resin was used to purify the gene. The
The concentration of adhP was determined by measuring the absorbance of
the E2 at 280nm. The concentration was measured to be 0.12mg/mL, which was
similar to the concentration measured by the Bradford assay, which was
0.17mg/mL. The differences
Study Guide: Biochemistry
Hydrophilic vs Hydrophobic. Since biological chemistry occurs largely in an aqueous environment, the
interaction of a biological molecule with water is very important. That interaction is influenced by two primary
1. Bacterial motors differ from eukaryotic ones in that the bacterial motor
moves along a polymeric track.
moves when ATP is hydrolyzed.
spins around a central axis.
A and B.
A, B, and C.
Ans: C Section: 35.4
2. What is the energy source us
1. Energy used to propel molecular motors includes
Ans: D Section: Introduction
A and B.
A, B, and C.
2. The major family(ies) of eukaryotic motor proteins is (are)
A) myosins. B) kinesins. C) dynei
Use the following to answer questions 1-10:
Choose the best answer from the list below. Not all of the answers will be used.
f) sugar-phosphate units
1 How can recombinant DNA technology aid in protein purification?
Ans: Large quantities of proteins can be expressed, allowing for extensive characterization of
the protein. Affinity tags can be fused to the protein to aid in solid phase studies. Mature