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MCB 3416, Fall 2008 September 18, 2008
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Routine & challenging features of forensic casework 1. Forensic samples of minimal quantity. 2. Mixed samples.
-Conditions that evidentiary samples are often exposed to can degrade DNA. -Degradation affects loci differently based upo
STR Analysis is Performed by Comparing Size of Questioned Peak to Allelic Ladders
z What are STRs and how are they amplified z STR applications in forensics
Capillary electrophoresis Analysis of DNA data using Genema
Flourescent Short Tandem Repeat (STR) Analysis
Michael T. Bourke, Ph.D.
zWhat are STRs and how are they amplified zSTR applications in forensics
Capillary electrophoresis Analysis of DNA data using Genemapper
Steps in Forensic DNA analysis
1) Extract DNA from biological stain 2) Evaluate DNA quality and approximate quantity (agarose yield gel) 3) Quantitate the amount of Human DNA in extract 4) Amplify DNA using STR genotyping kit/s 5) Separate and Detect ampl
MCB 3416 Forensic Application of DNA Science
Dr. Carll Ladd - Instructor Dr. Michael Bourke Instructor Bo Pietraszkiewicz (TA) Ranyelle Craig (TA)
Forensic methods of DNA typing STRs, mitochondrial, and Y-Chromosome Electropherogram Inte
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Single Nucleotide Polymorphisms (SNPs)
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the repeat region is variable between samples while the flanking regions where PCR primers bind are constant
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Forensic DNA Overview
Forensic Science: the application of natural sciences to matters of the law.
Carll Ladd Supervisor, DNA Unit
The recognition, identification, individualization, and evaluation of physical evidence
STATE AND NATIONAL DNA DATABASES: CODIS
Division Scientific Services CT Forensic Science Laboratory
Dr. Michael Bourke 203-639-6441 [email protected]
How Does it Work?
Convicted Offender Database Forensic Database
Compares the DNA profiles fro