1. The affinity of Stanfordin for the IEC column matrix is less than the affinity of NDase for
the IEC column matrix.
2. The cation in the KOAc buffer that is exchanging with the NDase on the column matrix
causing NDases elution is Potassium.
3. NDase has
1. BME, -mercaptoethanol, breaks covalent bonds while SDS, sodium dodecylsulfate,
breaks down peptide bonds.
2. There is only one polypeptide chain that comprises a protein with no quaternary structure.
3. You have to check for bubbles at the electrodes o
1a. The mean column fraction number used for the hemoglobin elution volume is 4.894736842,
the majority being from the 4th fraction.
1b. The mean column fraction number used for Vitamin B12 elution volume is 10.63157895, the
majority being from the 11th f
BIOS 11161 IN-LAB Thought Questions
Complete and review during lab today
1. List several functions that SDS-PAGE serves for the biological researcher.
a Serves to separate proteins
b Find molecular weights
c Finding differences in proteins from differen
Carboxyl cannot lend positive, can only lend negative
Deprotonated goes from neutral to negative
Above ph 4 carboxyl lends negative charge
Amino cannot lend negative
The amino group is not deprotonated until high pH goes from + to neutral charge
Watch Videos on Sakai and do Practice Problems
Cell Cycle and Mitosis: (Chapter 11), Lecture 8
Purpose of mitosis (why do cells divide?)
Increases body size during growth (development)
Replacement of dead or worn out cells
Basis of asexual reproduction in
Photosystems & Chemiosmosis- The Mechanism of ATP Synthesis in
The thylakoid membrane is composed of a phospholipid bilayer (color phospholipids "B" light bl
the kinds of
NO HELP ?
1. Large numbers of ribosomes are
present in cells that specialize in
2. Motor proteins are responsible for
intracellular movement by interacting
with what types of cellular structures?
3. Name 3 structures both prokaryotic
and eukaryotic cell
Analyzing the Free Energy of Reactions
Increasing order or
Anabolic or catabolic?
Change in G positive or
Spontaneous or coupled
Viral diseases (cont)
Bacterial diseases I
April 14, 2016
Viral hemmorrhagic fevers
Hantavirus, Ebola, Marburg,
Humans not natural host
Dont rely on humans for transmission
High mortality rates
Overall vascular system is damaged
Individual Preparation for Spring 2016 Registration
The First Year Curriculum
To receive a degree from Notre Dame, you must complete a minimum
of 60 credit-hours at Notre Dame and a minimum of
Worm diseases (cont.)
Microbes are good
April 26, 2016
We will finish Contagion first today.
Loa loa worm transmitted by deer flies
Adult worms live in eyes-microfilaria in blood
MCOB Overview of Requirements
Class of 2019
UNIVERSITY REQUIREMENTS FOR ALL BUSINESS STUDENTS
# of courses
Writing and Rhetoric
Type of organism that causes this disease (bacteria, virus, protozoa)
a. Viral infection
2. Eastern equine encephalitis
a. Viral infection
3. Western equine encephalitis
a. Viral infection
4. Venezuelan equine encephalitis
Video: Smallpox: Deadly again?
How long has smallpox been around (approximately)?
The beginning of civilization
What happens inside the body in a smallpox infection?
Destroys lungs and vital organs. People basically drown in their own fluids
Vaccines: Calling the Shots
How many parents in the United States immunize (or vaccinate) their children? What is the concern
More than 90%. The concerns are how many vaccines to give the children at once
could potentially dangerous
Video: Bird Flu: How safe are we?
What was the life expectancy in Botswana before and after AIDS?
Before AIDS 60
After AIDS below 40
How is Botswana different from many other sub-Saharan African countries?
It had the best chance of beating AIDS
RECOMMENDED FOUR-YEAR PROGRAM (YG 2019 and Later)
Video: Surviving AIDS
March 29, 2016
How/why did Bob Massie get the AIDS virus?
When was the AIDS virus discovered?
What type of virus is the AIDS virus? What is the importance of this in terms of an
Learning outcomes After studying this chapter, you should be able to:
. Distinguish among elements, compounds, and mixtures. (Section 1.2) Demonstrate the use Of Significant figuresl scientific notation, and
SI units in calculations. (Secti
To Get to Harvard, Go to Haiti? - The New York Times
8/16/16, 11:11 AM
To Get to Harvard, Go to Haiti?
AUG. 13, 2016
This summer, as last, Dylan Hernandez, 17, noticed a theme on the social
Measuring the Immeasurable | Partners In Health
9/10/15, 10:28 AM
Discourse & tools for global health professionals:
Knowledge Center (http:/www.pih.org/knowledge-center)
DONATE NOW (/DONATE)
Partners InOur Story (http:/www.pih.o
Four Traditions of Philanthropy*
Central to the history of philanthropy in the United States is a vision of human
connectedness. As Ellen Condliffe Lagemann has written, American philanthropy represents a
long history of ef
CHEMISTRY 10181 Introduction to Chemical Principles
Lecture: MWF, 10:30 11:20 am, Jordan 101
Tutorial: W, 3:30-4:20 pm, Jordan 105
Professor Jon P. Camden
Office: 140E McCourtney Hall
Office Hours: T, 1:30-3:00 pm, TBD
Learning Resource Center
At the Learning Resource Center, located in room 228 of the Coleman-Morse Center, students
can take part in collaborative learning sessions or small group tutoring sessions with a student
leader who has previously taken the course
Cells Tissues Organs 2007;185:4047
Signal Transduction Pathways Involved in
Epithelial-Mesenchymal Transition in Oral Cancer
Compared with Other Cancers
Julia Yu Fong Chang a, b John M. Wright a Kathy K.H. Svoboda b