Microbial Diseases of the Digestive System
Structure and Function of the Digestive System
Gastrointestinal (GI) tract or alimentary canal
Small and large intestine
Teeth and tongue
BIO125 INTRO. MICROBIOLOGY
Never enter the lab, use microscopes, or perform dissections unless your
instructor is present.
Always learn the location of exits and safety equipment when working in the
Mannitol Salt Agar
Mannitol salt agar (MSA) has a 7.5% salt concentration. This inhibits all growth except Staphylococcus
Stapylococcus aureus reacts with the mannitol in the agar and we see this reaction as a yellow color
change. There is a pH
Examination of Living Microorganisms
Handling and Examining Cultures
Viewing Bacteria in the Living State
The simplest method for examining living microorganisms is to suspend
them in fluid and prepare a wet mount.
Exercise #5, 7, 8, 9, 10
Preparation of smears and Staining
Allow you to determine if a microorganism is present.
If a microorganism is present, you can determine its shape.
You CANNOT tell if it is gram positive or negative with a
Exercise 45, 46 & 47
Bacteria of the Skin, Bacteria of the Respiratory Tract,
Bacteria of the Mouth
Bacteria of the Skin
Primarily made up of Staphylococci and Propionobacterium
There are other organisms like Streptococci and Corynebacterium
We have tw
Identification system of Enterobacteriaceae
and other gram negative, oxidase negative bacteria.
EnteroPluri-Test is a 12-sector system containing special culture media that permits identification of the
Mannitol salt agar (MSA), EMB, and Hemolysis
Mannitol Salt Agar (MSA)
NaCl-7.5%-inhibits Gram (-) bacteria
S. aureus produces the enzyme mannitol dehydrogenase, which breaks down
mannitol into pyruvate which ferments to produ
How to identify Enterobacteriacae (enteric microbiota)
(there is an additional pdf that is from the manufacturer)
Enterotubes are made by numerous manufacturers
So what does an enterotube contain?
How do we interpret the results?
Okay, now we
Microbial Diseases of the Respiratory System
Structure and Function of the Respiratory System
Upper respiratory system
Nose, pharynx, middle ear, and eustachian tubes
Saliva and tears protect mucosal surfaces
Lower respiratory system
Enterotube lab 4/19/16
Organisms used: _
What is the purpose of setting up an Enterotube test?
What are some of the compartments in the Enterotube covered with to generate Anaerobic conditions?
Which end of the enterotube is the handle end of the tube