BI0 140 Enzymes
Introduction: The purpose of this lab was to observe enzymes. Enzymes are a special class of
proteins that are involved in chemical reactions within the body.
Step 1: Obtained the items needed
BI0 140 Observing Bacteria, Protists, and Fungi
Introduction: The purpose of this lab was to observe different organisms from Kingdom Bacteria,
Kingdom Protista, and Kingdom Fungi. Bacteria is something that is found in
BI0 140 Introduction to Microscopes
Introduction: The purpose of this lab was to learn about the Binocular Compound Light
Microscope and how to properly use it. This type of microscope can be used to look at things
BI0 140 Mitosis investigation
Introduction: The purpose of this lab was to observe mitosis in an onion root tip and a fish.
Mitosis is the division of the cells nucleus but mitosis is commonly used synonymously with the
BI0 140 Observing Eukaryotic Cells
Introduction: The purpose of this lab was to observe some eukaryotic cells. A eukaryotic cell
contains a nucleus, mitochondria and chloroplasts. Eukaryotic cells also often contain flag
BI0 140 Cellular Respiration and Photosynthesis
Introduction: The purpose of this lab was to observe alcohol fermentation as well as
photosynthesis. Alcohol fermentation is a process in which sugars get converted into ce
BI0 140 Genetics and Blood typing; Reproduction and Development
Introduction: The purpose of this lab was test a victims blood along with some blood found at
the crime scene and four suspects blood. We also observed some
BI0 140 Chemical Components of living things
Introduction: The purpose of this lab was to learn how chemicals react
Step 1: plugged in hot plate and put beaker with water in it on hot plate to
Enterotube lab 4/19/16
Organisms used: _
What is the purpose of setting up an Enterotube test?
What are some of the compartments in the Enterotube covered with to generate Anaerobic conditions?
Which end of the enterotube is the handle end of the tube
How to identify Enterobacteriacae (enteric microbiota)
(there is an additional pdf that is from the manufacturer)
Enterotubes are made by numerous manufacturers
So what does an enterotube contain?
How do we interpret the results?
Okay, now we
Mannitol salt agar (MSA), EMB, and Hemolysis
Mannitol Salt Agar (MSA)
NaCl-7.5%-inhibits Gram (-) bacteria
S. aureus produces the enzyme mannitol dehydrogenase, which breaks down
mannitol into pyruvate which ferments to produ
Identification system of Enterobacteriaceae
and other gram negative, oxidase negative bacteria.
EnteroPluri-Test is a 12-sector system containing special culture media that permits identification of the
Exercise 45, 46 & 47
Bacteria of the Skin, Bacteria of the Respiratory Tract,
Bacteria of the Mouth
Bacteria of the Skin
Primarily made up of Staphylococci and Propionobacterium
There are other organisms like Streptococci and Corynebacterium
We have tw
Exercise #5, 7, 8, 9, 10
Preparation of smears and Staining
Allow you to determine if a microorganism is present.
If a microorganism is present, you can determine its shape.
You CANNOT tell if it is gram positive or negative with a
Examination of Living Microorganisms
Handling and Examining Cultures
Viewing Bacteria in the Living State
The simplest method for examining living microorganisms is to suspend
them in fluid and prepare a wet mount.
Mannitol Salt Agar
Mannitol salt agar (MSA) has a 7.5% salt concentration. This inhibits all growth except Staphylococcus
Stapylococcus aureus reacts with the mannitol in the agar and we see this reaction as a yellow color
change. There is a pH
BIO125 INTRO. MICROBIOLOGY
Never enter the lab, use microscopes, or perform dissections unless your
instructor is present.
Always learn the location of exits and safety equipment when working in the