Questions Test #3
Phage typing is used:
For identifying bacterial strains within one specie
What is CORRECT for the hybridization with a probe?
o It is used to separate RNA from DNA
o Probe carries a fluorescent dye
Used to detect specific nucleot
What is Genetics?
The science of heredity
Research in Genetics takes place on several levels
Where genetic material can be found?
Genetic information is contained within genetic material DN
How to Identify the Disease?
Direct testing: microscopic, immunological, genetic methods. Few minutes
Cultivation of microorganisms: few days to few weeks (Mycobacterium tuberculosis)
Based on symptoms (athletes foot)
MCB 2000 Question Test #2
Genetic Material in Prokaryotes is contained in:
Both Plasmids and Nucleoid
DNA nucleotides are composed of:
Deoxyribose sugar, Phosphate group and Bases.
Which of the enzymes are NOT involved in DNA replication:
By: Jenique Murphy, Lesly Mendez, and Gidnie Dort
Influenza also known as The Flu
The most recognized disease in the
United States other than the common
Viral disease of the lower respiratory
Hemagglutinin (HA) spike
Whooping Cough and Its
Return to the US
What is Whooping Cough?
Pertussis or whooping cough is a respiratory illness caused by a type
of bacteria calledBordetella pertussis.
Pertussis is only found in humans & spreads from by coughing or
Name: Shawna G.
Water Bacteriology Experiment # 8 Lab Report # 3
Please answer the following using the data collected and any other additional
1. Is the Completed Test positive or negative for lactose fermentation? Explain your answer.
BY SHAWNA G
What is Septicemia?
Septicemia is a serious infection in
which a bacterial infection elsewhere
in the body enters the bloodstream.
Early aggressive treatment does
increase the chance of one
Notes on Hamburger Bacteriology. Experiment # 9
The purpose of the lab is to identify the presence (or lack )of different bacteria on different
meat samples that can cause food poisoning and spoilage as well as possibly identify if the
Shawna G UO1
Water Bacteriology Lab Report # 2
Using all the data collected so far (Presumptive and Confirmative Tests) answer the
1. Was the Confirmative test positive or negative for fecal coliforms (Escherichia
coli)? Explain your answer for
Shawna G UO1
Questions for Lab Report 1 of Experiment # 8 (attach to
the Data sheet)
What type of bacteria predominates in the lake water tested? Based your answer in all the
data collected from Gram Staining, MPN and other tests (Coliforms, Pseudomonas)
The Purpose of a simple stain is to be able to recognize the three basic shapes of bacteria.
Obtain dry clean glass slides.
Heat the slide by passing it through quickly a Bunsen burner flame.
Mark each section with waxing
Examination of Natural Microbes
To prepare student to familiarize themselves with the lab equipment such as a microscope, square glass,
cover slips, ect. To enable them to discern and identify the microbes as bacteria, algae, fungi, or protozoa
The Purpose of this lab was to observe the life cycle and mating behavior of the
TA will shake and
provide two tube
Students will take one
drop from minus strain
and place on microsope
Differentiate the antigenic classification of antibodies
Light chain: There are two classes of L chains that are designated kappa and
lambda. In any immunoglobulin molecule, there will be found either two kappa
or two lambda chains, but never both.
Lab Experiment 9
Chemotaxis of Gram-negative species
The purpose of the experiment is to observe bacteria chemotaxis of Gram-negative species with a
repellant and an attractant. We compared the number of bacteria
Lab Experiment 10 Bacteriophages from Nature
The purpose of this experiment was to isolate coliphages through six serial 1:10 dilutions from
FIU sewage by determining the phage titer in sewage onto six Nutrient A
Lab Experiment 5
Isolation of Bacteria: Viable Titer and Pure Culture
The purpose of this experiment is to isolate pure cultures and obtain viable titers in three lab
sessions. First, we serially diluted a soil s
Lab Experiment 3
The Gram Stain
The purpose of this experiment was to fix living pathogenic bacteria, yeast and our household
sample to slides using heat-fixed smear. This made the slides safe and allowed the org
Berlinda Lisa Juste
Lab Experiment 6
Phenotype Determination: What is the Phenotype of Your Bacteria?
The purpose of this experiment was to determine the biochemical traits of our unknown
bacterium isolated from a nutrient slan
Lab Experiment 4
The Spore Stain and The Acid-Fast Stain
The purpose of this experiment was to perform a spore stain on two cultures of Bacillus sp. as
well as an acid-fast stain on cultures of Mycobacterium sp.
Lab Experiment 8
Mating of Chlamydomonas reinhardi
The purpose of this experiment is to observe the sexual reproduction life cycle of
Chlamydomonas reinhardi for 5 minute intervals for 30 minutes using the mating
Lab Experiment 7
The purpose of this experiment was to collect our choice of bacterium, facultative anaerobe
Lactobacillus rhamnosus, from the suspect environment as per PowerPoint presentation
Lab Experiment 2
The Winogradsky Column
The purpose of this experiment is to enrich for aerobic and anaerobic photosynthetic bacteria,
and sulfur cycle bacteria using the Winogradsky column. The column contains m
How to Use a Compound Microscope
Lab Experiment 1
The purpose of the lab is to observe microbes such as bacteria, yeasts, algae and protozoa under
the compound microscope as was duplicated three hundred years ago
Introduction to Microbiology
What is a microorganism?
an organism (or virus) too small to be seen w/ naked eye. (smaller than .5 mm).
Where do we find them?
everywhere. air, soil, water, objects, inside the body, etc.
Perception of microbes
Infectious Diseases Affecting the Nervous System
Structure of the nervous system
Divided into two parts:
Central nervous system (CNS)
Brain and Spinal Cord
Peripheral nervous system (PNS)
Nerves that transfer commands between the CNS and muscle
Chapter 12 Antimicrobial Drugs
Chemical substances used for treatment of infectious diseases - chemotherapy
Antimicrobial drugs have selective toxicity (harmful against microbes and not the host)
Antibiotic substance produced by one mi
Specific Defenses of the Host
When the non-specific defense fails, then the third, specific line of immunity is activated
Immunity is not innate but adaptive; it is acquired over time
It is characterized with a specificity and mem