Concentration of diluted solutions of Copper
MiVi = MfVf
8 mL Copper and 2 mL Water
(0.1 M)(8 mL Cu) = (Mf)(10 mL Cu)
(0.1 M )(8 mL Cu)
(10 mL Cu)
= 0.08 M
6 mL Copper and 4 mL water
(0.1 M)(6 mL Cu) = (Mf)(10 mL Cu)
(0.1 M )(6 mL Cu)
(10 mL C
Name: _ Section: _ Date: _
Homework - Readings
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population as well as conserv
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Sorting Proteins into Transition Vesicles:
How can you sort things to go into the right vesicle and know what is in the vesicle.
Take a transmembrane protein that have receptors on both sides.
Receptor on the lumen and the cytoplasm side.
There are lots of filaments in nuclear matrix and cytoskeleton
But there are probably some kind of difference.
o So to study the difference, you would just get a isolated nuclear matrix fraction.
How to get nuclear matrix:
Marching through the EM system, we went through the RER, went through the golgi, and after
coming out of the golgi, you can either go to the plasma membrane or you can go to lysosome.
Lysosomes: Acidic organelles for degradation and recycling.
Use of Centrifugation to Separate organelles, vesicles and other things.
Centrifugation can be used to separate organelles, and other things.
Spinning at different speeds
o Separation based primarily on size and weight.
Division of a single cell.
Two main considerations:
Nuclear Division: Mitosis.
When cell divides, first thing it has to do is divide the nuclear contain.
Take the DNA split it in half, and then go into cell division.
Three possibilities for DNA replication in bacteria.
Set up three rational possibility for DNA replication.
Experiment are done to test out the experiments.
o The data will help determine which hypothesis makes sense.
o When you have do
Oxidized Compound (wants electrons)
o Reducing agent needed, it donates electrons; Oxidized compound will accept
electrons and protons.
Once it accepts the electron, it becomes a reduced compound. (Gets the
o A compound in its o