Subculturing Venus Flytrap onto Multiplication Medium
Objective: Students will transfer stage I Venus Flytrap plants to stage II multiplication medium.
Dionaea muscipula is commonly called Venus Flytrap. It is a perennial carnivorous member of
Objective: Students will prepare media for upcoming experiments
Tobacco (hormone effects) Media
Add per 500 mL
Benzyladenine (BA) (stock)
indole acetic acid (I
Control of organogenesis in cultures of Nicotiana tabacum
Objective: To observe the effects of various levels of hormones on tobacco explants.
1. You will be given a container with tissue from one or more sterile tobacco
seedling and plat
Objective: Students will become familiar with steps involved in citrus transformation using
Citrus Transformation Protocol:
A) 28 to ? days before experiment initiation:
1. Sterilize seeds of the desi
The GUS gene is used in transgenic studies to verify the transmission of the transformation vector into the
target plant. It is informative because nothing in the plant tissue would stain blue; the blue stain can only
mean the pl
DNA Extraction and PCR
DNA Extraction Qiagen DNeasy Plant Mini Kit
1. Using a Mortar & Pestle, grind the tissue in a small quantity of Liquid Nitrogen. Pour tissue into a
sterile 2ml tube.
2. Add 400uL Buffer AP1 and 4uL RNase A. Vortex and incubat
Potato Microtuber Production
Establishing stock potato shoots
Potato tubers have been sprouted in water. Cut off a potato shoot (approximately 3-5cm in
Rinse shoot tips in water for 10 minutes
Surface disinfest in 20% commercial bleach fo
Protocol for Anther Culture
Tobacco is the ideal material for anther culture. So the basic protocol described below should be
applicable to anther culture in general with modifications. The immature anthers containing
uninucleate pollen grains at
Student will transform tobacco from sterile explants.
1. Start Agrobacterium culture to be used from a glycerol stock. Grow up overnight at 28C,
under the proper selection.
2. On the next day cut the leaf pieces a