Today's Lecture
1) Rabi Molecular Beam experiment (continued from last time) 2) Fri, Oct 4: Behavior of nuclear spins in a magnetic field II a. Bloch equations b. Phenomenological introduction to T1 and T2 c. RF Pulses d. "Teach Spin" apparatus
A. S. Edis
Today's Lecture
8) Monday, Oct 16: Introduction to 2D NMR a. Two pulse exchange experiment (aka NOESY) b. COSY for homework
A. S. Edison University of Florida 2006
1D NMR
For a single spin and no coupling, a 1D NMR experiment can be described as follows.
Todays Lecture
8) Monday, Oct 19: Introduction to 2D NMR a. Two pulse exchange experiment (aka NOESY) b. COSY for homework
A. S. Edison University of Florida 2009
1D NMR
For a single spin and no coupling, a 1D NMR experiment can be described as follows. R
Today's Lecture
8) Wed, Oct 18: Bloch Equations and Relaxation a. Transverse and longitudinal magnitization b. Mechanisms of relaxation c. Measuring relaxation
October 1, 2004
Joanna R. Long
1
Individual magnetic moments:
B0
Bulk Magnetization:
B0
M
^
Today's Lecture
9) Wed, Oct 6: Interactions of NMR in Solution and Solids a. Chemical shift b. J-coupling c. Dipole-dipole interactions d. Quadrupolar interactions e. Solid state NMR experimental considerations
Review articles on ssNMR: Angewandte Chemie
Today's Lecture
10) Mon, Oct. 23: Relaxation Measurements a) Correlation functions b) Causes of relaxation c) NMR measurements of correlation functions d) Example of relaxation measurements
Consider random field fluctuations along the X axis:
Lets monitor
Today's Lecture
11) Wed, Oct 25: Driving interactions between spins a. Heteronuclear interactions in solution b. Heteronuclear interactions in solids c. Improving sensitivity d. Improving resolution
What do we mean by heteronuclear interactions?
Interacti
Today's Lecture
11) Fri, Oct 27: 2D NMR Review and Summary a. 2D NMR b. NOESY c. Other Important biological experiments d. Heteronuclear correlations
A. S. Edison University of Florida
1
FT in t will give 2D frequency spectrum
f2
2D Exchange NMR t2
FT of
Today's Lecture
13) Mon, Oct 30: Assignments: I a. Important homonuclear (e.g. 1H) experiments b. Small molecules c. Peptide assignments
A. S. Edison University of Florida 2006
Homonuclear 2D Experiments
Name COSY Correlated Spectroscopy Type of correlati
Today's Lecture
4) Wed, Oct 14: Data Collection a. Digitization and Spectral Width b. Fourier Transform c. Quadrature detection
A. S. Edison University of Florida 2009
What happens next?
1) A sample with a single resonance (e.g. water) is placed in a stro
Today's Lecture
6) Mon, Oct 14: Product operators II a. Scalar (J) coupling b. Multiple pulse experiments
A. S. Edison University of Florida 2006
Last lecture we discussed rotations of bulk magnetization.
z
r1r x Mx = r0r y r r r0r z r r x0x x My = x1x y
Today's Lecture
6) Fri, Oct 10: Product operators II a. Scalar (J) coupling b. Multiple pulse experiments
A. S. Edison University of Florida 2008
J coupling and chemical shift example
All of the splittings are J couplings
H NMR 1D spectra of Methyl -D-Ara
Today's Lecture
1) Rabi Molecular Beam experiment (continued from last time) 2) Fri, Oct 3: Behavior of nuclear spins in a magnetic field II a. Bloch equations b. Phenomenological introduction to T1 and T2 c. RF Pulses d. "Teach Spin" apparatus
A. S. Edis
Today's Lecture
3) Wed, Oct 4: Introduction to NMR Parameters a. Review of Bloch equations b. Chemical shift-BMRB database c. J coupling-Karplus equation d. T1 e. T2
A. S. Edison University of Florida 2006
Bloch Equations
dM z (t ) M (t ) - M 0 r r = M x
Today's Lecture
3) Mon, Oct 6: Introduction to NMR Parameters a. Review of Bloch equations b. Chemical shift-BMRB database c. J coupling-Karplus equation d. T1 e. T2
A. S. Edison University of Florida 2008
Bloch Equations
dM z (t ) M (t ) - M 0 r r = M x
Today's Lecture
4) Wed, Oct 9: Data Collection a. Digitization and Spectral Width b. Fourier Transform c. Quadrature detection
A. S. Edison University of Florida 2006
What happens next?
1) A sample with a single resonance (e.g. water) is placed in a stron
Todays Lecture
4) Fri, Oct 9: Product operators I (tools to simplify the quantum mechanics) a. RF pulses b. Chemical shift
A. S. Edison University of Florida 2009
Weve really just been talking about rotations most of the time!
NMR is all rotations.
A. S.
Today's Lecture
5) Wed, Oct 8: Product operators I (tools to simplify the quantum mechanics) a. RF pulses b. Chemical shift
Download Mathematica Player and Bloch Equation demo http:/demonstrations.wolfram.com/MagneticResonanceAndBlochEquations/
A. S. Edis
Today's Lecture
5) Wed, Oct 11: Product operators I (tools to simplify the quantum mechanics) a. RF pulses b. Chemical shift
A. S. Edison University of Florida 2006
We've really just been talking about rotations most of the time!
NMR is all rotations.
A.
Todays Lecture
6) Mon, Oct 14: Product operators II a. Scalar (J) coupling b. Multiple pulse experiments
A. S. Edison University of Florida 2009
Last lecture we discussed rotations of bulk magnetization.
z
1 x Mx = 0 y 0 z 0 x My = 1 y 0 z 0 x Mz = 0 y 1
Today's Lecture
14) Wed, Nov 1: Assignments: II a.Important 2D heteronuclear experiments b.3D NMR c.Assignment strategies in proteins d.Intro to protein structure determination
A. S. Edison University of Florida 2006
1
H-based Peptide Resonance Assignment