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Transport by vesicles
From ER to:
Figure 15-5 Essential Cell Biology ( Garland Science 2010)
Protein sorting within the cell
Eukaryotic cells have separate
compartments to separate
Lectures 10 and 11
Cytoskeleton - A System of intracellular filaments
mitosis - helps pull chromosomes apart and carry out cytokinesis
trafficking of organelles, vesicles
Recombinant DNA Technology
We can now rapidly
sequence entire genomes
Our understanding of genes began in 1970s
with the ability to isolate specific genes
This has allowed us to understand gene
function, influenced our unders
Cell Biology Exam 3-Transcription
Study online at quizlet.com/_hywcy
About how many microRNA genes are currently known to be encoded in the human genome, and what can these genes do?:
+400; miRNA assemble with special protein ca
Chapter 4 Notes Cell Biology
Active site region on the surface of an enzyme that binds to a substrate molecule and catalyzes
its chemical transformation.
Allosteric describe a protein that can exist in multiple conformations depending on the binding
Chapter 1 Notes
Cells - small, membrane-enclosed units filled with a concentrated aqueous solution of chemicals
& endowed with the extraordinary ability to create copies of themselves by growing and
then dividing into two.
cells are n
Energetics and Kinetics
Why do we care?
Energetic and kinetic analyses of biological processes provide
- a means to quantitatively evaluate their specific roles
- physical and mechanistic insights
- the information necessary to develop predictiv
PCB3023, Spring 2016, SV
What is cell biology?
Cell biology is the study of cell structure, dynamics and
It is a scientific discipline that studies cells, their physiological
properties, their structure, the organelles
Chapter 2 Notes Cell Biology
Acid- a molecule that releases a proton when dissolved in water; this dissociation generates
hydronium (H3O+) ions, thereby lowering the pH. Higher concentration of hydronium ions, the
more acid the solution.
Transcription- study questions
1. Briefly describe the central dogma.
- DNA does not synthesize proteins by itself. DNA is copied into RNA, this segment is a gene.
The resulting RNA copies are used to direct the synthesis of proteins. Therefore, flow of
Chapter 3 Cell Biology
Acetyl CoA acetyl coenzyme A activated carrier that donates the carbon atoms in its readily
transferable acetyl group to many metabolic reactions, including the citric acid cycles and fatty
acid biosynthesis; the acetyl group is lin
Intracellular Transport I
1. What are the major cellular organelles and briefly describe their functions.
Nucleus DNA & RNA synthesis
ER- synthesis of lipids, synthesis of proteins sent to organelles or plasma membrane, CA2+
released for cell s
Transport by vesicles: endocytosis, exocytosis and lysosomal degradation
1. Describe the process of endocytosis by clathrin coated pits. Include cargo, cargo receptor,
adaptin, and clathrin in your answer.
1) clathrin proteins start to assemble like a bas
Gene Regulation Study Questions
1. What makes different cell types different?
- they all contain different proteins that encode for different functions
- transcription factors
- gene expression
2. Describe the concept of cloning an animal from a fibroblas
Protein targeting to the ER
- Review from last time
Protein targeting to the ER lumen
Figure 15-15 Essential Cell Biology ( Garland Science 2010)
Insertion of single-pass transmembrane protein
into ER membrane
Figure 15-16 Essential Cell Biology ( Garland
1. During translation, RNA is made into Protein.
2. There are 4 nucleotides and 20 amino acids.
3. What is the genetic code?
Each of the 20 AAs found in proteins is designated by a triple codon in mRNA.
4. How was the genetic code solved?
Molecular biology techniques study guide
1. What are restriction enzymes, and what is their purpose in bacteria? How are they a useful
- Cut DNA molecules at specific sites, they protect bacteria from bacteriophage
- Also used in recombinant DNA tec
DURING CELL DIVISION AND
Nucleotide sequence that codes for RNA
RNAs can be divided into four main types
mRNA (messenger RNA)
tRNA (transfer RNA)
rRNA (ribosomal RNA)
sRNA (small RNA)
THE REPLICATION OF DNA
It has not escaped our notice that the
specific pairing we have postulated
immediately suggests a possible copying
mechanism for the genetic material.
J. D. Watson and F. H. C. Crick, 1953
Population genetics is the study of
the genetic composition of a
population and the forces that
determine and change that
Let us begin with a few definitions
Species = A group of organisms
Final Exam- December 6, 2015 12:30-2:00pm
50 Questions: 40 multiple choice and 10 T/F questions.
1. Genomic Imprinting- form of gene regulation where the offspring expresses the copy of a gene
from ONE parent, but not both.
i. We inhe
Mitosis asexual mother cell to 2 daughters, same # of chromosomes as mother cell.
Meiosis daughter cells have # chromosomes as mother cell, sexual reproduction.
Gametes with half # of chromosomes unite during fertilization.
FINAL EXAM- December 6, 2015-SUNDAY @ 12:30 PM-2:00 PM
50 Questions: 40 multiple choice; 10 T/F
Genomic imprinting-form of gene regulation where the offspring expresses the copy of a gene
from ONE parent, but not both.
We inherit t
Lab Report #1: Determining the Unknown Genotype of D. melanogaster through Chi
Lab Partners: Bretty Aziz, Michael Matli & Cody Nulph
Methods and Materials:
Setting Up a Genetic Cross:
Lab Report #2: Cloning and Sequencing an 18S Gene derived from a D. melanogaster
Partner: Michael Matli
The purpose of the experiments performed was to be able to successfully clone and