Ex1-3: Common Aseptic transfers and Inoculation Methods
GOAL: The goal of this lab is to learn to inoculate and transfer cultures
aseptically to avoid contamination.
4. Sterile media
1. Bunsen burner
Chapter 1 Chemical Bonds and Water
1Q1 A carbon atom contains six protons and six neutrons. What are its atomic number,
atomic weight and how many electrons does it have? How many additional electrons
must it add to fill its outermost shell?
1Q2 How is an
Valence shell electron pair repulsion (VSEPR) theory the ideal angles between bonding and
nonbonding groups of electrons.
Tetrahedral bond angle is 109.5 degrees.
S orbital is shaped spherical and the P orbitals are tear shaped lobes that intersect at the
Chapter 3.1 Protein Structure
3.1Q1 Draw the structure of a peptide bond.
3.1Q2 What are the four weak non-covalent interactions that determine the
conformation of a protein?
3.1Q3 What are the major properties that distinguish different amino acids from
Chapter 3 Protein Structure
Proteins are polypeptides, long linear chain of amino acids linked by peptide bonds. Major
macromolecule in interior of cell. Catalyzing metabolic reaction, DNA replication, responding to
stimuli, transporting molecule from one
Chapter 3.2 Protein Function
3.2Q1 Why are the membrane-spanning regions of transmembrane proteins frequently
3.2Q2 Provide a detailed and complete description of a typical protein-binding site
interacting with its ligand (also substrate, a
Chapter 4 Membrane Transport
A phospholipid molecule has dual characteristics of hydrophobic and hydrophilic.
Structure and Properties of Lipid Bilayer
Phospholipid bilayer caused by the hydrophobic effect. Hydrophobic patches come into contact
Cell and Molecular Biology (CHAPTER 1)
Atoms and electron shells. Covalent bonds. Ionic bonds. Organic molecules.
What molecules are made up of?
Which bonds creates charges?
What are organic molecules?
Hydrogen bonds, lattice structures,
Cell and Molecular Biology
Chapter 2: Structural Outline of Biological Chemicals
Carbohydrates are made up of only C,H and O.
Carbohydrates are found in biological systems as monosaccharides, disaccharides, and
oligosaccharides, and polysacc
Chapter 4.1 Membranes & Transport
4.1Q1 Why do membrane lipids form a bilayer?
4.1Q2 What are the differences of micelles and lipid bilayers?
4.1Q3 What is the role of cholesterol in membrane bilayers?
4.1Q4 What defines a transmembrane domain in proteins
Chapter 5.1: Energy Transformations in Cells
5.1Q1 Describe briefly the three stages of cellular metabolism that lead from food to waste
products in animal cells.
5.1Q2 Animals and plants use oxidation to extract energy from food molecules. Explain
Chapter 11, pages 253-263
carried out by chemolithotrophs.
chemolithotrophs (and any other microorganism) need electrons,
ATP, and Carbon source
electrons released from energy s
Fermentations and the
catabolism of polymers
Chapter 11 pages 248-253
oxidation of NADH produced by glycolysis
pyruvate or derivative used as endogenous
electron acceptor (i.e. get reduced)
Chapters 3 and 13 Catalysis, the Use of Energy
by Cells and How Cells Obtain Energy from Food
3.1 In the stepwise conversion of methane to carbon dioxide via the formation of methanol,
formaldehyde and formic acid as intermediates, descri
Chapter 4 Protein Function
4.15 Why are the membrane-spanning regions of transmembrane proteins frequently alphahelical?
4.16 Given that proteins act as molecular machines explain why conformational changes are so
important in protein fun
Chapter 4 - Protein Structure
4.1 Draw the general structure of an amino acid.
4.2 Draw the structure of a peptide bond.
4.3 What are the four weak non-covalent interactions that determine the conformation of a
4.4 What are the m
Chapters 12 and 15 - Transport and Compartments
12.1 Describe the types of molecules that diffuse through membranes without any specific aid.
Thinking on the molecules Na+, O2, H2O, K+ and CO2, order them based on their ability to
Chapter 2- Molecules in Cells
2.13 How glucose, mannose and galactose differ from each other?
2.14 If 0.5 mole of glucose weighs 90 g, what is the molecular weight of glucose?
2.15 What is the concentration, in grams per liter (g/l), of a
Chapter 14 - Photosynthesis and Energy Metabolism
14.1 Describe the membrane structure and compartments of chloroplasts and mitochondria
14.2 How does ATP couple reactions? What are the essential underlining chemical and physical
Chapters 11 Membranes
11.1 What are the differences of micelles and lipid bilayers?
11.2 Describe the types of lipid movements that occur at lipid bilayers. Explain the type of
movement that happens less often too.
11.3 What is the role o
Chapter 2- Chemical Components
2.1 If the isotope 32S has 16 protons and 16 neutrons, how many protons and how many neutrons
will the isotope 35S have?
2.2 A carbon atom contains six protons and six neutrons. What are its atomic number, a