Harvard-MIT Division of Health Sciences and Technology
HST.071: Human Reproductive Biology
Course Director: Professor Henry Klapholz
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HST-071 FINAL EXAMINATION
You are asked to see a young male with th
You have a crude lysate sample (CL) containing a mixture of six proteins (1, 2, 3, 4, 5, galactosidase), and your goal is to obtain purified -gal. Some characteristics of these
proteins are shown in the table below:
Solution Key - 7.016 Problem Set 4
Arginine biosynthesis is an example of multi-step biochemical pathway where each step is catalyzed
by a specific enzyme (E1, E2 and E3) as is outlined below.
Your supervisor gives you t
2004 FINAL FOR STUDY FALL 2005
a. 10 points The technique of in situ hybridization was used to examine the
expression of the Evl gene. Explain the principal of this technique. Indicate how
the different Evl RNA isoforms were detected.