Zhang Problem Set 1
a. During development of multicellular organisms the fate of millions of cells
needs to be determined. Transcription factors can determine cell fate by activating
a specific subset of genes available in the genome. Since transcripti
Zhang Problem Set 4
In the Drosophila embryo, the 3 untranslated regions (3 UTRs) of the mRNAs
[the regions between the translation-termination codons and the poly(A) tails] are
responsible for localizing bcd and nos to the anterior and posterior poles
BIOL335 Problem Set #2
1. Assume that E. coli can use the alternative sugar cellobiose, and that the cellobiase enzyme (which
splits cellobiose into two glucose molecules) is only made in high amounts when cellobiose
/ Lecture 12-13: Pattern Formation in Drosophila embryogenesis
What are maternaleffect and zygotic lethal mutants? What types pattern defects are observed among
the maternaleffect embryo-lethal mutants?
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Lecture 14: Zygotic Embryo Lethal Patterning Genes
What are the phenotypes of zygotic embryo lethal patterning mutants? [035 ()4 1C LXKhC mum-m +5
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/ Lecture 15: Gap gene function and the control of pair rule and homeotic selector genes
What is the expression pattern of pair rule genes and how is the pattern established by the gap genes?
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An F plasmid that carries the pro+ allele is discovered in a strain of E. coli. The
plasmid is conjugated into a strain that is pro . Most of the recipients mobilize the F and
the allele to F cells at epidemic frequency, but occasionally colonies are f
Biol 335 Problem Set #1 Answer Guide:
Textbook Qs Chapter 5:
19. Describe the state of the F factor in an Hfr, F+, and F strain. Answer: An Hfr strain has the fertility factor F integrated into the chromoso
Week 3 Answers:
Problem Set #3:
b) No. We are not given any information on the orientation of either gene
c.) and d.)
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Chapter 10 Answers:
14.) Why is ligase nee
Lecture 10: Somatic Mutation and Cancer
Explain how mutations can result in Cancer.
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Molecular markers-use in genetic
Transposable genetic elements
Genetic linkage review for
molecular marker analysis
Genetics of Development
! Development-orderly sequence of change
leading to increased complexity.
! this process requires precise orchestration
of gene expression.
! no apparent permanent change in the
genetic material but
BIOL335 Molecular Genetics Lecture Notes
Lecture 3: 9 Jan. 2015
Liquid media or solid support media (addition of agar: solid support similar to gelatin).
Spread suspension on petri plate with agar gel: incubate 1 to 2 days: division b
BIOL335 Lecture Notes
Lecture 15: 11 Feb. 2015
Prokaryotic Gene Regulation: all or nothing turn on to get gene product.
Complex Regulatory Regions Enable Fine-Tuning of Gene Expression
Each gene can have many regulatory proteins:
In humans, ~2000 genes e
Lecture 2: Developmental Genetics of Multicellular Organisms 0
Understand basic concepts of development.
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JULY 22ND LECTURE NOTES ON METHODS OF CLONING A GENE
Change a single nucleotide at a time then assay for function
Once youve cloned and sequenced a gene You can design the short primer that incorporates the
single nucleotide mutation or multiple mutation