Thermodynamics, with its emphasis on heat engines and abstract energy concepts,
has often seemed irrelevant to biochemists. Indeed, a conventional introduction to
the subject is almost certain to convince the student that m
310L490] imam 5/11/04
NAME- ,_ SSH;
>X(/4Ipts) When will the absorbance of a protein be negative?
1:. w extinction coefcient is negative
. when the protein is too concentrated for the Spectrophotometer (Le. an absmbanoe > 5)
BIOL 4001 EXAM l 2 March 11, 2014
I. (3pts) Under what conditions will the Hill coefcient for ligand binding be negative?
a. when nu-bar is used instead of Y-bar
b. n more than one ligand binds to the protein
. when binding is negatively cooper
Table A.1 Useful physicai constants .
Speed of light
Permittivity of vacuum
Thermodynamics and Statistical Thermodynamics
All of chemistry, including the chemistry of biological systems, can be viewed as being based
on the interrelated physical factors of energetics, structure, and dynamics. In some ways,
Lecture 6: Thermodynamics of Ion and
Thermodynamics of Ion Transport
Transport of ions across biological membranes
Applications of standard potentials
Electron transfer in bioenerget
Lecture 7: The Rates of Reactions
The Rates of Reactions
Some of the earlier steps in photosynthesis may take
place in about 1-100 ps
The binding of a neurotransmitter can have an effect
after about 1 s
Folding of a ne
PROBLEM SET #1
1. One definition of work is force times distance (F x l). If a constant force of 104 dynes acts to
move an object by 100 cm, what is the work done in ergs? in joules? in calories?
2. If a system comprised of only gas decreases in
PROBLEM SET #2
Several of the following questions pertain to the graphs provided with the problem set.
1. In DSC scan #1, what are the molar enthalpy (H) and molar heat capacity (Cp) changes for
unfolding of this protein?
2. In DSC scan #2, the
wiluw % :1
[calf g/ 4/
M Min: A 2* 0
A f e/
* M I.
' o 1/ gun! ca
g = MW
its cfw_and (3 _
/,38' X/O'z J7;
g =- L3 IL 40
'-= I. 6 ? A /0 '25 M
: 6? 77 506. 5C7 ' Fm r MA 4 cW/f)
Some properties of a system:
Some other ararerties
Table 2.E Heat capacities ofsome materi